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PI-103 sensitizes acute myeloid leukemia stem cells to daunorubicin-induced cytotoxicity
- Source :
- Medical oncology (Northwood, London, England). 30(1)
- Publication Year :
- 2012
-
Abstract
- To date, acute myeloid leukemia (AML) shows very poor outcome for conventional chemotherapy. Leukemia stem cells (LSCs) are insensitive to conventional chemotherapeutic drugs and play a central role in the pathogenesis of AML. Failure to effectively ablate these cells may lead to AML relapse following chemotherapy. Phosphatidylinositol 3-kinase (PI3K)/Akt/mammalian target of rapamycin (mTOR) signaling pathway is constructively activated in LSCs. This pathway can be inhibited by PI-103, a novel synthesized molecule of the pyridofuropyrimidine class, resulting in the apoptosis of LSCs. Therefore, we investigate the influences of PI-103 in combination with daunorubicin (DNR) on the LSCs. Our data indicate that PI-103 synergistically sensitizes LSCs to DNR-induced cytotoxicity. In addition, the PI-103/DNR co-treatment can induce significant apoptosis in LSCs, but sparing hematopoietic stem cells. The synergistic effect and the LSCs-specific apoptosis mechanism may be associated with the inhibition of PI3K/Akt/mTOR signaling pathway. Our results suggest that PI-103 in combination with DNR may be a potent and less toxic therapy for targeting LSCs and deserve further preclinical and clinical studies in the treatment of AML.
- Subjects :
- Adult
Male
Cancer Research
Myeloid
Adolescent
Daunorubicin
Cell Survival
Pyridines
Blotting, Western
Apoptosis
Pharmacology
Young Adult
Antineoplastic Combined Chemotherapy Protocols
medicine
Humans
Furans
Protein kinase B
PI3K/AKT/mTOR pathway
Cells, Cultured
Aged
Aged, 80 and over
business.industry
Myeloid leukemia
Hematology
General Medicine
Middle Aged
medicine.disease
Flow Cytometry
Haematopoiesis
Leukemia
Leukemia, Myeloid, Acute
medicine.anatomical_structure
Pyrimidines
Oncology
Neoplastic Stem Cells
Female
Stem cell
business
medicine.drug
Signal Transduction
Subjects
Details
- ISSN :
- 1559131X
- Volume :
- 30
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Medical oncology (Northwood, London, England)
- Accession number :
- edsair.doi.dedup.....a4dad9267631ec22ad3025c631ece45e