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Functional requirements of protein kinases and phosphatases in the development of the Drosophila melanogaster wing
- Source :
- G3: Genes, Genomes, Genetics, Vol 11, Iss 12 (2021), G3: Genes|Genomes|Genetics, Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2021
- Publisher :
- Oxford University Press, 2021.
-
Abstract
- Protein kinases and phosphatases constitute a large family of conserved enzymes that control a variety of biological processes by regulating the phosphorylation state of target proteins. They play fundamental regulatory roles during cell cycle progression and signaling, among other key aspects of multicellular development. The complement of protein kinases and phosphatases includes approximately 326 members in Drosophila, and they have been the subject of several functional screens searching for novel components of signaling pathways and regulators of cell division and survival. These approaches have been carried out mostly in cell cultures using RNA interference to evaluate the contribution of each protein in different functional assays and have contributed significantly to assign specific roles to the corresponding genes. In this work, we describe the results of an evaluation of the Drosophila complement of kinases and phosphatases using the wing as a system to identify their functional requirements in vivo. We also describe the results of several modifying screens aiming to identify among the set of protein kinases and phosphatases additional components or regulators of the activities of the epidermal growth factor and insulin receptors signaling pathways.<br />PGC2018-094476-B-100 from the Secretaría del Estado de Investigación, Desarrollo e Innovación. The CBMSO enjoys institutional support from the Fundación Ramón Areces and Fundación Banco de Santander.
- Subjects :
- AcademicSubjects/SCI01140
wing morphogenesis
AcademicSubjects/SCI00010
Phosphatase
Biology
QH426-470
Genetic screen
AcademicSubjects/SCI01180
RNA interference
Genetics
Animals
Drosophila Proteins
Wings, Animal
Phosphorylation
Molecular Biology
Gene
Genetics (clinical)
Kinase
phosphorylation
Mutant Screen Report
biology.organism_classification
Wing morphogenesis
Phosphoric Monoester Hydrolases
Cell biology
Drosophila melanogaster
genetic screen
RNAi
AcademicSubjects/SCI00960
RNA Interference
Signal transduction
Protein Kinases
Subjects
Details
- Language :
- English
- ISSN :
- 21601836
- Volume :
- 11
- Issue :
- 12
- Database :
- OpenAIRE
- Journal :
- G3: Genes, Genomes, Genetics
- Accession number :
- edsair.doi.dedup.....a4eca9fa52d014d3606f0432a81807d4