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Different Sarcocystis spp. are present in bovine eosinophilic myositis
- Source :
- Veterinary Parasitology. 197:543-548
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- It has been suggested that Sarcocystis species are associated with bovine eosinophilic myositis (BEM). To date, parasite identification in this myopathy has been based on morphological techniques. The aim of the present study was to use molecular techniques to identify Sarcocystis species inside lesions of BEM. Histologically, BEM lesions of 97 condemned carcasses were examined for the presence of Sarcocystis species. Intralesional and extralesional cysts were collected using laser capture microdissection and the species was determined with a PCR-based technique based on 18S rDNA. Intralesional sarcocysts or remnants were found in BEM lesions in 28% of the carcasses. The majority (82%) of intralesional Sarcocystis species were found to be S. hominis. However S. cruzi and S. hirsuta were also found, as well as an unidentified species. It can be concluded that Sarcocystis species present in lesions of BEM are not restricted to one species.
- Subjects :
- Pathology
medicine.medical_specialty
Sarcocystosis
Molecular Sequence Data
Cattle Diseases
Biology
Species Specificity
medicine
Animals
18s rdna
Myopathy
Phylogeny
Myositis
Laser capture microdissection
Base Sequence
General Veterinary
Sarcocystis
General Medicine
DNA, Protozoan
medicine.disease
biology.organism_classification
Sarcocystis species
Bovine eosinophilic myositis
Cattle
Parasitology
medicine.symptom
Subjects
Details
- ISSN :
- 03044017
- Volume :
- 197
- Database :
- OpenAIRE
- Journal :
- Veterinary Parasitology
- Accession number :
- edsair.doi.dedup.....a5005dbf197b1110aebf346098de1d83