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Human oocyte maturation in vitro is improved by co-culture with cumulus cells from mature oocytes
Human oocyte maturation in vitro is improved by co-culture with cumulus cells from mature oocytes
- Source :
- Reproductive BioMedicine Online. 36:508-523
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The conventional method of human oocyte maturation in vitro in the presence of gonadotrophins continues to be a relatively low-success procedure in the assisted conception programme owing to suboptimal maturation conditions in the absence of an ovarian 'niche' and poor understanding of this procedure at the molecular level in oocytes. In this study, the gene expression profiles of human oocytes were analysed according to their manner of maturation: in vivo (in the ovaries) or in vitro (matured either by the conventional method or by a new approach - co-cultured with cumulus cells of mature oocytes from the same patient). Our results show that the in-vitro maturation procedure strongly affects the gene expression profile of human oocytes, including several genes involved in transcriptional regulation, embryogenesis, epigenetics, development, and the cell cycle. The in-vitro maturation of oocytes co-cultured with cumulus cells from mature oocytes provides an ovarian 'niche' to some degree, which improves oocyte maturation rates and their gene expression profile to the extent that they are more comparable to oocytes that naturally mature in the ovarian follicle.
- Subjects :
- 0301 basic medicine
Embryonic Development
Biology
03 medical and health sciences
0302 clinical medicine
Gene expression
Transcriptional regulation
medicine
Humans
Epigenetics
Ovarian follicle
Gene
Principal Component Analysis
Cumulus Cells
030219 obstetrics & reproductive medicine
Gene Expression Profiling
Embryogenesis
Obstetrics and Gynecology
Oocyte
Coculture Techniques
In Vitro Oocyte Maturation Techniques
In vitro maturation
Cell biology
030104 developmental biology
medicine.anatomical_structure
Reproductive Medicine
Oocytes
Female
Developmental Biology
Subjects
Details
- ISSN :
- 14726483
- Volume :
- 36
- Database :
- OpenAIRE
- Journal :
- Reproductive BioMedicine Online
- Accession number :
- edsair.doi.dedup.....a5173e8bd7bcc2782fabcbd69e2fa10c
- Full Text :
- https://doi.org/10.1016/j.rbmo.2018.01.011