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A label-free, ultra-highly sensitive and multiplexed SERS nanoplasmonic biosensor for miRNA detection using a head-flocked gold nanopillar
- Source :
- The Analyst. 144:1768-1776
- Publication Year :
- 2019
- Publisher :
- Royal Society of Chemistry (RSC), 2019.
-
Abstract
- Cell-free micro-ribonucleic acids (cf-miRNAs) regulate most human genes by controlling the mRNA translation and have been considered as the most promising biomarkers for cancer diagnosis and prognosis. Due to their low abundance, short length, and similar sequences, an attractive approach that is ultrasensitive and label-free is highly demanded. Herein, we develop a label-free, ultra-high sensitivity and selectivity multiplex miRNA using surface-enhanced Raman scattering to detect cancer-associated miRNAs with head-flocked gold nanopillars as a substrate. Using a complementary DNA probe platform and an ultra-high sensitivity SERS based biosensor, we achieved high selectivity in targeting miRNAs with extremely low detection limits, without any chemical or enzymatic reactions and evaluated the correlation between the miRNA expression level and the Raman signal intensity. A reproducible SERS signal is monitored via uniformly fabricated elastic head-flocked gold nanopillars through a mapping method. This system supports the detection of cell-free miRNAs in blood, which are utilized and quantified as biomarkers to diagnose and provide a prognosis for cancers and intractable diseases. In the foreseeable future, this advanced label-free miRNA detection system is highly promising for a less tumor-invasive biopsy for the early diagnosis and prognosis of cancer.
- Subjects :
- Metal Nanoparticles
Nanotechnology
Biosensing Techniques
02 engineering and technology
Spectrum Analysis, Raman
01 natural sciences
Biochemistry
Analytical Chemistry
Limit of Detection
microRNA
Biomarkers, Tumor
Electrochemistry
Humans
Environmental Chemistry
Multiplex
Spectroscopy
Label free
Nanopillar
Detection limit
Chemistry
010401 analytical chemistry
Nucleic Acid Hybridization
DNA
021001 nanoscience & nanotechnology
0104 chemical sciences
Highly sensitive
MicroRNAs
MCF-7 Cells
Gold
Signal intensity
DNA Probes
0210 nano-technology
Biosensor
Subjects
Details
- ISSN :
- 13645528 and 00032654
- Volume :
- 144
- Database :
- OpenAIRE
- Journal :
- The Analyst
- Accession number :
- edsair.doi.dedup.....a518db611ec9ac31c110950e73c74d65