Back to Search
Start Over
LINC00313 alleviates osteoarthritis progression in mice through promoting TRAF1 promoter methylation and inhibiting the ASK1/JNK signaling pathway
- Source :
- Immunopharmacology and Immunotoxicology. 44:732-745
- Publication Year :
- 2022
- Publisher :
- Informa UK Limited, 2022.
-
Abstract
- This study aimed to explore the underlying role and mechanism of LINC00313 in osteoarthritis (OA) progression. CHON-001 chondrocytes were treated with interleukin (IL)-1β to induce OA in vitro, and then transfected with LINC00313 overexpression plasmids (pcDNA-LINC00313) or small interfering RNA against tumor necrosis factor (TNF) receptor-associated factor 1 (si-TRAF1). Cell viability, apoptosis, levels of inflammatory cytokines tumor necrosis factor-α (TNF-α), IL-6 and IL-8, and expression of extracellular matrix (ECM) degradation related proteins in CHON-001 cells were determined. TRAF1 promoter methylation were was detected with methylation‐specific polymerase chain reaction (MSP) assay. Furthermore, a c-Jun N-terminal kinase (JNK) signaling activator was used to confirm whether the apoptosis signal-regulating kinase 1 (ASK1)/JNK signaling pathway was involved in the function of LINC00313/TRAF1 axis in chondrocytes. In addition, an OA mouse model was established and lentivirus LINC00313 overexpression vector (Lv-LINC00313) was injected, and then inflammatory cytokine levels, ECM protein expression, and pathological changes in cartilage tissues were detected. LINC00313 was downregulated and TRAF1 was upregulated in OA cartilage tissues. LINC00313 overexpression or TRAF1 silencing attenuated IL-1β-induced viability inhibition, apoptosis, inflammation and ECM degradation in CHON-001 cells. Moreover, LINC00313 inhibited TRAF1 expression through promoting DNA methyltransferase 1 (DNMT1) mediated promoter methylation. TRAF1 overexpression reversed the effects of LINC00313 on IL-1β-induced chondrocyte injury. LINC00313 overexpression inhibited the ASK1/JNK signaling pathway, and JNK activator reversed the effect. In addition, Lv-LINC00313 treatment alleviated cartilage tissue damage and cartilage matrix degradation in OA mice. LINC00313 alleviated OA progression through inhibiting TRAF1 expression and the ASK1/JNK signaling pathway.
- Subjects :
- Pharmacology
Interleukin-6
MAP Kinase Signaling System
Tumor Necrosis Factor-alpha
Interleukin-1beta
Interleukin-8
Immunology
JNK Mitogen-Activated Protein Kinases
Apoptosis
DNA
Methyltransferases
General Medicine
MAP Kinase Kinase Kinase 5
Toxicology
Methylation
TNF Receptor-Associated Factor 1
Mice
Osteoarthritis
Animals
Immunology and Allergy
RNA, Small Interfering
Subjects
Details
- ISSN :
- 15322513 and 08923973
- Volume :
- 44
- Database :
- OpenAIRE
- Journal :
- Immunopharmacology and Immunotoxicology
- Accession number :
- edsair.doi.dedup.....a538675b933d64811fe1c030813b2bc2