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Expression of CD82 in human trophoblast and its role in trophoblast invasion
- Source :
- PLoS ONE, Vol 7, Iss 6, p e38487 (2012), PLoS ONE
- Publication Year :
- 2012
- Publisher :
- Public Library of Science (PLoS), 2012.
-
Abstract
- Background Well-controlled trophoblast invasion at maternal-fetal interface is a critical event for the normal development of placenta. CD82 is a member of transmembrane 4 superfamily, which showed important role in inhibiting tumor cell invasion and migration. We surmised that CD82 are participates in trophoblast differentiation during placenta development. Methodology/Principal Findings CD82 was found to be strongly expressed in human first trimester placental villous and extravillous trophoblast cells as well as in trophoblast cell lines. To investigate whether CD82 plays a role in trophoblast invasion and migration, we further utilized human villous explants culture model on matrigel and invasion/migration assay of trophoblast cell line HTR8/SVneo. CD82 siRNA significantly promoted outgrowth of villous explants in vitro (P
- Subjects :
- Embryology
Immunofluorescence
Fluorescent Antibody Technique
lcsh:Medicine
Matrix metalloproteinase
Kangai-1 Protein
Biochemistry
RNA interference
Cell Movement
Pregnancy
Molecular Cell Biology
Signaling in Cellular Processes
lcsh:Science
reproductive and urinary physiology
Multidisciplinary
Reverse Transcriptase Polymerase Chain Reaction
Decidua
Obstetrics and Gynecology
Cell Differentiation
Cell migration
Immunohistochemistry
Trophoblasts
Cell biology
Nucleic acids
medicine.anatomical_structure
embryonic structures
Medicine
Female
Cell Movement Signaling
Research Article
Signal Transduction
Blotting, Western
Immunology
In Vitro Techniques
Biology
Cell Line
Placenta
medicine
Humans
Cell Proliferation
Matrigel
Cell growth
lcsh:R
Proteins
Trophoblast
Molecular biology
Transmembrane Proteins
Cell culture
Immunologic Techniques
RNA
lcsh:Q
Developmental Biology
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 7
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a53c6d3d6802c2e4b00ceefce65f29ba