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Simultaneous Determination of Seven Phenolic Acids in Rat Plasma Using UHPLC-ESI-MS/MS after Oral Administration of Echinacea purpurea Extract
- Source :
- Molecules; Volume 22; Issue 9; Pages: 1494, Molecules, Vol 22, Iss 9, p 1494 (2017), Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
- Publication Year :
- 2017
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2017.
-
Abstract
- A rapid and sensitive Ultra High Performance Liquid Chromatography Electrospray Ionization Tandem Mass Spectrometry (UHPLC-ESI-MS/MS) method was developed and validated to simultaneously determine the concentration of seven phenolic acids (syringic acid, ferulic acid, caffeic acid, vanillic acid, p-coumaric acid, 3,4-dihydroxybenzoic acid and 4-hydroxybenzoic acid) in rat plasma after oral administration of Echinacea purpurea extract. After mixing with the internal standard (IS), butylparaben, plasma samples were prepared by liquid–liquid extraction with ethyl acetate. The separation was performed using the Agilent Eclipse Plus C18 column (1.8 μm, 2.1 mm × 50 mm) with a gradient system consisting of solution A (0.1% acetic acid in water) and solution B (methanol) at a flow rate of 0.3 mL/min. The detection was accomplished by a multiple reaction monitoring (MRM) mode with electrospray ionization (ESI). The method was validated in terms of linearity, precision, accuracy, extraction recovery, matrix effect and stability. This method was successfully applied to study the pharmacokinetic properties of the seven compounds after oral administration of Echinacea purpurea extract in rats.
- Subjects :
- Male
Electrospray ionization
Liquid-Liquid Extraction
Ethyl acetate
Pharmaceutical Science
Administration, Oral
Parabens
01 natural sciences
Article
Echinacea
Analytical Chemistry
Ferulic acid
Rats, Sprague-Dawley
lcsh:QD241-441
Acetic acid
chemistry.chemical_compound
lcsh:Organic chemistry
Limit of Detection
Tandem Mass Spectrometry
Drug Discovery
Vanillic acid
Caffeic acid
Hydroxybenzoates
Animals
Humans
Physical and Theoretical Chemistry
Chromatography, High Pressure Liquid
Echinacea purpurea
Chromatography
010405 organic chemistry
Plant Extracts
010401 analytical chemistry
Organic Chemistry
Selected reaction monitoring
Syringic acid
0104 chemical sciences
Rats
phenolic acids
UHPLC-ESI-MS/MS
pharmacokinetics
chemistry
Chemistry (miscellaneous)
Molecular Medicine
Subjects
Details
- Language :
- English
- ISSN :
- 14203049
- Database :
- OpenAIRE
- Journal :
- Molecules; Volume 22; Issue 9; Pages: 1494
- Accession number :
- edsair.doi.dedup.....a5777e4151d0bb4f23dafd732db9dec6
- Full Text :
- https://doi.org/10.3390/molecules22091494