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Calcium electroporation in three cell lines: a comparison of bleomycin and calcium, calcium compounds, and pulsing conditions
- Source :
- Frandsen, S K, Gissel, H, Hojman, P, Eriksen, J & Gehl, J 2013, ' Calcium electroporation in three cell lines; a comparison of bleomycin and calcium, calcium compounds, and pulsing conditions ', BBA General Subjects . https://doi.org/10.1016/j.bbagen.2013.12.003
- Publication Year :
- 2014
- Publisher :
- Elsevier BV, 2014.
-
Abstract
- Electroporation with calcium (calcium electroporation) can induce ATP depletion-associated cellular death. In the clinical setting, the cytotoxic drug bleomycin is currently used with electroporation (electrochemotherapy) for palliative treatment of tumors. Calcium electroporation offers several advantages over standard treatment options: calcium is inexpensive and may readily be applied without special precautions, as is the case with cytostatic drugs. Therefore, details on the use of calcium electroporation are essential for carrying out clinical trials comparing calcium electroporation and electrochemotherapy.The effects of calcium electroporation and bleomycin electroporation (alone or in combination) were compared in three different cell lines (DC-3F, transformed Chinese hamster lung fibroblast; K-562, human leukemia; and murine Lewis Lung Carcinoma). Furthermore, the effects of electrical pulsing parameters and calcium compound on treatment efficacy were determined.Electroporation with either calcium or bleomycin significantly reduced cell survival (p0.0001), without evidence of a synergistic effect. Cellular death following calcium or bleomycin treatment occurred at similar applied voltages, suggesting that similar parameters should be applied. At equimolar concentrations, calcium chloride and calcium glubionate resulted in comparable decreases in cell viability.Calcium electroporation and bleomycin electroporation significantly reduce cell survival at similar applied voltage parameters. The effect of calcium electroporation is independent of calcium compound.This study strongly supports the use of calcium electroporation as a potential cancer therapy and the results may aid in future clinical trials.
- Subjects :
- Electrochemotherapy
Biophysics
chemistry.chemical_element
Calcium
Biology
Pharmacology
Bleomycin
Biochemistry
Carcinoma, Lewis Lung
Mice
chemistry.chemical_compound
Cricetulus
Cricetinae
medicine
Animals
Humans
Fibroblast
Molecular Biology
Antibiotics, Antineoplastic
Electroporation
Lewis lung carcinoma
Calcium Compounds
Molecular biology
medicine.anatomical_structure
chemistry
Cell culture
K562 Cells
Subjects
Details
- ISSN :
- 03044165
- Volume :
- 1840
- Database :
- OpenAIRE
- Journal :
- Biochimica et Biophysica Acta (BBA) - General Subjects
- Accession number :
- edsair.doi.dedup.....a57d0309918fdfd865dbc665ba6c6a99