Back to Search
Start Over
A new fluorescent staining method for callose of microspore mother cells during meiosis
- Source :
- Biotechnic & Histochemistry. 87:300-302
- Publication Year :
- 2012
- Publisher :
- Informa UK Limited, 2012.
-
Abstract
- To observe the dynamic behavior of callose of microspore mother cells during meiosis, we developed a convenient, rapid and efficient staining method using an improved carbol fuchsin/aniline blue solution. The stained microspore mother cells during meiosis showed yellowish green callose, red cytoplasm and dark red chromosomes when excited with blue light, which produced a contrasting image with a three-dimensional effect. When stained with only improved carbol fuchsin solution, the cells had red cytoplasm and chromosomes when excited with green light. The improved carbol fuchsin solution can be used to replace other more expensive DNA-specific dyes, such as DAPI and H33258, to reduce experimental costs.
- Subjects :
- Aniline Compounds
Time Factors
Histology
Moeller stain
Staining and Labeling
Callose
ALIZARIN RED
General Medicine
Biology
Molecular biology
Staining
Meiosis
Medical Laboratory Technology
chemistry.chemical_compound
chemistry
Microspore
Cytoplasm
Rosaniline Dyes
Pollen
Carbol fuchsin
Glucans
Fluorescent Dyes
Subjects
Details
- ISSN :
- 14737760 and 10520295
- Volume :
- 87
- Database :
- OpenAIRE
- Journal :
- Biotechnic & Histochemistry
- Accession number :
- edsair.doi.dedup.....a58b6c94a6f61f7929a93f3365ac17b7