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Molecular characterization of glucosinolates and carotenoid biosynthetic genes in Chinese cabbage ( Brassica rapa L. ssp. pekinensis )
- Source :
- Saudi Journal of Biological Sciences, Vol 25, Iss 1, Pp 71-82 (2018)
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- The present study aimed to investigate the contents of glucosinolates (GSLs) and carotenoids in eleven varieties of Chinese cabbage in relation to the expression level of the important transcription factors. MS and HPLC analysis identified the presence of 13 GSLs (progoitrin, sinigrin, glucoalyssin, gluconapoleiferin, gluconapin, glucocochlearin, glucobrassicanapin, glucoerucin, 4-hydroxyglucobrassicin, glucobrassicin, 4-methoxyglucobrassicin, neoglucobrassicin and gluconasturtiin) and four carotenoids (lutein, zeaxanthin, α-carotene and β-carotene). GSL contents were varied among the different cabbage varieties. The total GSL content ranged from 2.7 to 57.88μmol/gDW. The proportion of gluconapin (54%) and glucobrassicanapin (22%) was higher in all the varieties, respectively. Results documented the variation in total and individual carotenoid contents that have also been observed among different varieties; however, the total carotenoid contents ranged from 289.12 to 1001.41mgkg−1DW (mean 467.66). Interestingly, the proportion of lutein (66.5) and β-carotene (25.9) were higher than α-carotene (5.1) and zeaxanthin (2.5%). Consequently, the expression level of the regulatory gene, MYB28 was higher in ‘K0648’ and was directly proportional to GSL content. Similarly, the expression levels of 1-PSY were higher in ‘K0112’; however, the expression levels of 2-ZDS, 3-LCYB, 4-LCYE, 5-CHXB and 7-NCED genes showed no significant difference. In addition, the correlation between GSL and carotenoid contents and gene expression level showed moderate significant difference in each Chinese cabbage.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Lutein
Glucosinolates
Biology
01 natural sciences
Article
Gluconasturtiin
Glucobrassicin
03 medical and health sciences
chemistry.chemical_compound
Botany
Brassica rapa
Food science
lcsh:QH301-705.5
Carotenoid
Progoitrin
chemistry.chemical_classification
Agricultural and Biological Sciences(all)
Chinese cabbage
Carotenoids
Zeaxanthin
030104 developmental biology
lcsh:Biology (General)
chemistry
Sinigrin
Gene expression
HPLC
General Agricultural and Biological Sciences
010606 plant biology & botany
Subjects
Details
- ISSN :
- 1319562X
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Saudi Journal of Biological Sciences
- Accession number :
- edsair.doi.dedup.....a5983a9a07693b92a7864d6320071bcf