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Ethylenediaminetetraacetic Acid Disodium Salt Acts as an Antifungal Candidate Molecule against Fusarium graminearum by Inhibiting DON Biosynthesis and Chitin Synthase Activity
- Source :
- Toxins, Vol 13, Iss 17, p 17 (2021), Toxins, Volume 13, Issue 1
- Publication Year :
- 2021
- Publisher :
- MDPI AG, 2021.
-
Abstract
- Fusarium fungi are the cause of an array of devastating diseases affecting yield losses and accumulating mycotoxins. Fungicides can be exploited against Fusarium and deoxynivalenol (DON) production. However, Fusarium resistance to common chemicals has become a therapeutic challenge worldwide, which indicates that new control agents carrying different mechanisms of action are desperately needed. Here, we found that a nonantibiotic drug, ethylenediaminetetraacetic acid disodium salt (EDTANa2), exhibited various antifungal activities against Fusarium species and DON biosynthesis. The infection of wheat seeding caused by F. graminearum was suppressed over 90% at 4 mM EDTANa2. A similar control effect was observed in field tests. Mycotoxin production assays showed DON production was significantly inhibited, 47% lower than the control, by 0.4 mM EDTANa2. In vitro experiments revealed a timely inhibition of H2O2 production as quickly as 4 h after amending cultures with EDTANa2 and the expression of several TRI genes significantly decreased. Chitin synthases of Fusarium were Mn2+-containing enzymes that were strongly inhibited by Mn2+ deficiency. EDTANa2 inhibited chitin synthesis and destroyed the cell wall and cytomembrane integrity of Fusarium, mainly via the chelation of Mn2+ by EDTANa2, and thus led to Mn deficiency in Fusarium cells. Taken together, these findings uncover the potential of EDTANa2 as a fungicide candidate to manage Fusarium head blight (FHB) and DON in agricultural production.
- Subjects :
- Fusarium
chitin synthases
Antifungal Agents
Health, Toxicology and Mutagenesis
deoxynivalenol
lcsh:Medicine
Ethylenediaminetetraacetic acid
Toxicology
Article
Microbiology
Cell wall
03 medical and health sciences
chemistry.chemical_compound
Chitin
Biosynthesis
Gene Expression Regulation, Fungal
Magnesium
Mycotoxin
Edetic Acid
030304 developmental biology
Calcium Chelating Agents
chemistry.chemical_classification
Chitin Synthase
0303 health sciences
Manganese
EDTANa2
biology
030306 microbiology
lcsh:R
food and beverages
biology.organism_classification
Fungicide
Enzyme
chemistry
manganese ion
Calcium
Trichothecenes
Subjects
Details
- Language :
- English
- ISSN :
- 20726651
- Volume :
- 13
- Issue :
- 17
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....a5a171892305c92a2803b106313726ff