Inositol Deficiency Induces Autophagy Impairing via PI3K/Akt/mTOR/p70S6K Signaling (P11-032-19)

OBJECTIVES: Our previous case-control study found that maternal MI deficiency was associated with an increased risk of NTDs. Bioinformatics analysis showed that PI3K/Akt/mTOR/p70S6K signaling pathway might be one of the important regulatory mechanisms of inositol deficiency-induced NTDs. So, we intended to explore the possible mechanisms of PI3K/Akt/mTOR/p70S6K signaling in inositol deficiency-induced neural tube defects (NTDs) in this study. METHODS: The activity of the PI3-kinase in MI deficiency NE-4C cells was detected by the PI3-kinase ELISA kit. Using the method of western blot, we analyzed the activity of the PI3K/Akt/mTore/p70S6K signaling pathway and the level of LC3B. And the LC3B levels were also detected by electron microscopy. RESULTS: The result showed that PI3K activity was significantly higher in the Li2CO3-treated group than in the control cells (P