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A Device for Ribbon Collection for Array Tomography with Scanning Electron Microscopy
- Source :
- Acta Histochemica et Cytochemica
- Publication Year :
- 2017
- Publisher :
- Japan Society of Histochemistry & Cytochemistry, 2017.
-
Abstract
- “Array tomography” is a method used to observe the fine structure of cells and tissues in a three-dimensional view. In this method, serial ultrathin sections in the ribbon state (ribbons) are mounted on a solid substrate and observed by scanning electron microscopy (SEM). The method may also be used in conjunction with post-embedding immunocytochemistry. However, it is difficult to mount many serial ribbons on a substrate manually. We developed an inexpensive laboratory-made device that mounts ribbons by pulling a nylon fishing line and lifting the substrate up from the water in a knife boat. Using this device, we succeeded in mounting several ribbons consisting a mean of 205.6 (SD: 37.7) serial ultrathin sections on 1.25 (SD: 0.06) × 1.25 (SD: 0.06)-cm silicon substrates. Furthermore, it was confirmed that our method is suitable for ribbons derived from water-soluble resin blocks. We were also able to stain the specimens by post-embedding immunocytochemistry. Thus, our method is useful in mounting numerus sections on a substrate for array tomography with SEM.
- Subjects :
- 0301 basic medicine
Histology
Materials science
Silicon
Physiology
Scanning electron microscope
Analytical chemistry
chemistry.chemical_element
Substrate (printing)
Biochemistry
Pathology and Forensic Medicine
03 medical and health sciences
0302 clinical medicine
Ribbon
post-embedding labeling
array tomography
serial ultrathin section
business.industry
three-dimensional observation
fungi
Scanning confocal electron microscopy
Cell Biology
body regions
Technical Advancement
030104 developmental biology
chemistry
Electron tomography
Scanning ion-conductance microscopy
Optoelectronics
sense organs
Tomography
business
scanning electron microscopy
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 13475800 and 00445991
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Acta Histochemica et Cytochemica
- Accession number :
- edsair.doi.dedup.....a5ce70ace55174c931fe385dc02e70b7