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A natural mutation in the Tyk2 pseudokinase domain underlies altered susceptibility of B10.Q/J mice to infection and autoimmunity
- Source :
- Proceedings of the National Academy of Sciences. 100:11594-11599
- Publication Year :
- 2003
- Publisher :
- Proceedings of the National Academy of Sciences, 2003.
-
Abstract
- The B10.Q/J strain of mice was serendipitously discovered to be highly susceptible to infection by the intracellular protozoan parasite,Toxoplasma gondiibut markedly resistant to induction of autoimmune arthritis. We have previously shown that the B10.Q/J phenotype is controlled by a single recessive locus and is associated with lymphocyte hyporesponsiveness to IL-12. Using genetic approaches, we have now localized the B10.Q/J locus to chromosome 9 and established its identity asTyk2, a Janus kinase essential for IL-12 and IFN-α/β cytokine signaling. The B10.Q/JTyk2gene contained a single missense mutation resulting in a nonconservative amino acid substitution (E775K) in an invariant motif of the pseudokinase (Janus kinase homology 2) domain. This mutation appeared to result in the absence of the B10.Q/J-encoded Tyk2 protein, despite presence ofTyk2-specific transcripts. Phenotypically, B10.Q/J cells were indistinguishable fromTyk2-deficient cells, showing impaired signaling and biologic responses to IL-12, IL-23, and type I IFNs. The analogous E782K mutant of human Tyk2 failed to restore IFN-α responsiveness inTyk2null 11,1 cells. Our results indicate a crucial role for Tyk2 in T helper 1-mediated protective and pathogenic immune responses. An additional implication of our findings is that naturally occurring mutations in theTyk2gene may underlie altered susceptibilities to infectious or autoimmune diseases in human and animal populations.
- Subjects :
- Mutant
Autoimmunity
Biology
Infections
Gene Expression Regulation, Enzymologic
Mice
Immune system
TYK2 Kinase
Animals
Point Mutation
Missense mutation
Genetic Predisposition to Disease
Gene
Genetics
Mice, Inbred BALB C
Multidisciplinary
Point mutation
Genetic Complementation Test
Proteins
Protein-Tyrosine Kinases
Biological Sciences
Molecular biology
Mutagenesis
Tyrosine kinase 2
Mutation
Janus kinase
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 100
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....a5f919de360eb4c1881e3baadbdcd61d