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Activity control of autodisplayed proteins on the same outer membrane layer of E. coli by using Z-domain/streptavidin/and lipase/foldase systems
- Source :
- Enzyme and Microbial Technology. 96:85-95
- Publication Year :
- 2017
- Publisher :
- Elsevier BV, 2017.
-
Abstract
- The autodisplay technology has been applied for expression of a desired protein on the outer membrane (OM) of Escherichia coli. In this work, the OM fractions of E. coli with two autodisplayed proteins were separately prepared and mixed to demonstrate the feasibility of control over the ratio of two autodisplayed proteins. As the first model, Z-domain and streptavidin were autodisplayed, and their activities were tested by means of the combined OM layer in a 96-well microplate and a surface plasmon resonance (SPR) biosensor. As the second model, lipase and foldase were autodisplayed which required an interaction between two proteins to obtain the activity of lipase. The OM fractions of E. coli with an autodisplayed lipase and foldase were separately prepared and mixed to demonstrate the feasibility of control over the ratio of two autodisplayed proteins when the interaction of two proteins is required within the same OM layer for the activity of the lipase.
- Subjects :
- 0301 basic medicine
Streptavidin
Recombinant Fusion Proteins
Bioengineering
02 engineering and technology
medicine.disease_cause
Applied Microbiology and Biotechnology
Biochemistry
03 medical and health sciences
chemistry.chemical_compound
Protein Domains
Escherichia coli
medicine
Lipase
Surface plasmon resonance
biology
Cell Membrane
021001 nanoscience & nanotechnology
030104 developmental biology
chemistry
Foldase
biology.protein
Cell Surface Display Techniques
0210 nano-technology
Bacterial outer membrane
Layer (electronics)
Biosensor
Biotechnology
Subjects
Details
- ISSN :
- 01410229
- Volume :
- 96
- Database :
- OpenAIRE
- Journal :
- Enzyme and Microbial Technology
- Accession number :
- edsair.doi.dedup.....a611357d217c5c66538c32339dee1340