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Novel RET Proto-oncogene variants identified in Turkish patients with thyroid carcinoma
Novel RET Proto-oncogene variants identified in Turkish patients with thyroid carcinoma
- Source :
- Gene. 746
- Publication Year :
- 2019
-
Abstract
- WOS: 000530711400001 PubMed: 32240776 Thyroid cancer is one of the few malignancies whose incidence is increasing in the last decades. Advances in understanding the molecular mechanisms lead to provide opportunity for prevention, effective early identification and targeted therapies for management. A total of 63 patients with participated in this study Genomic DNA samples were obtained from the samples formalin- embedded tissue and peripheral blood. Following polymerase chain reaction amplification of the 6 RET key exons (10, 11, 13, 14, 15, and 16) were applied and PCR products were subjected to next generation DNA sequencing (ABI 3730). Results revealed that; genotype frequencies were for rs1800961 (G > T) , GG 6 (%9.5), GT 17 (%27) TT40 (%63.5) for rs2472732 (G > A), GG31 (%49.2) GA29 (%46) AA3 (%4.8,) for rs1799939, (G > A) GG42 (%66.7) GA19 (%30.2) AA2 (%3.2), for rs1800962, (C > T) CC54 (%85.7) CT9 (%14.3), for rs1800863 (C > G), CC39 (%61.9) CG22 (%34.9) GG2 (%3.2), for rs3026272 (C > G) CC 13 (%20.6) CG 50 (%79.4). Additionally 15 potential novel genetic variants were identified in these key exons. Detailed information was given both known and new detected variants in supplementary table. Genetic variants distribution frequencies and new variants represented in Turkish thyroid cancer patients for RET proto-oncogene and that results would provide contribution to the literature. Ondokuz Mayis University BAPOndokuz Mayis University [PYO-TIP-1901.16.001] We would like to thank Ondokuz Mayis University BAP for supporting our project (PYO-TIP-1901.16.001).
- Subjects :
- 0301 basic medicine
Male
Genotype
Turkey
Biology
RET proto-oncogene
Proto-Oncogene Mas
DNA sequencing
Thyroid cancer
law.invention
Thyroid carcinoma
03 medical and health sciences
Exon
0302 clinical medicine
law
Genetics
medicine
Humans
Thyroid Neoplasms
Polymerase chain reaction
Next-generation DNA sequencing
Polymorphism, Genetic
Proto-Oncogene Proteins c-ret
General Medicine
Middle Aged
medicine.disease
Molecular biology
Genotype frequency
genomic DNA
030104 developmental biology
030220 oncology & carcinogenesis
RET protooncogene
Female
Subjects
Details
- ISSN :
- 18790038
- Volume :
- 746
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....a6716ffef9d54eb2c72f4f6e9c93c278