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Combined Enzymatic and Mechanical Cell Disruption and Lipid Extraction of Green Alga Neochloris oleoabundans
- Source :
- International Journal of Molecular Sciences, Vol 16, Iss 4, Pp 7707-7722 (2015), International Journal of Molecular Sciences, Volume 16, Issue 4, Pages 7707-7722
- Publication Year :
- 2015
- Publisher :
- MDPI AG, 2015.
-
Abstract
- Microalgal biodiesel is one of the most promising renewable fuels. The wet technique for lipids extraction has advantages over the dry method, such as energy-saving and shorter procedure. The cell disruption is a key factor in wet oil extraction to facilitate the intracellular oil release. Ultrasonication, high-pressure homogenization, enzymatic hydrolysis and the combination of enzymatic hydrolysis with high-pressure homogenization and ultrasonication were employed in this study to disrupt the cells of the microalga Neochloris oleoabundans. The cell disruption degree was investigated. The cell morphology before and after disruption was assessed with scanning and transmission electron microscopy. The energy requirements and the operation cost for wet cell disruption were also estimated. The highest disruption degree, up to 95.41%, assessed by accounting method was achieved by the combination of enzymatic hydrolysis and high-pressure homogenization. A lipid recovery of 92.6% was also obtained by the combined process. The combined process was found to be more efficient and economical compared with the individual process.
- Subjects :
- Microscopy, Electron, Scanning Transmission
Sonication
Cell morphology
ultrasonication
Homogenization (chemistry)
Article
Catalysis
lcsh:Chemistry
Inorganic Chemistry
Chlorophyta
Enzymatic hydrolysis
Cellulases
Ultrasonics
Physical and Theoretical Chemistry
lcsh:QH301-705.5
Molecular Biology
Spectroscopy
Biodiesel
Chromatography
biology
Hydrolysis
Organic Chemistry
high pressure homogenization
General Medicine
enzymatic lyses
Neochloris oleoabundans
biology.organism_classification
Lipids
microalga
Computer Science Applications
lcsh:Biology (General)
lcsh:QD1-999
Biofuels
Cell disruption
cell disruption
Intracellular
Peptide Hydrolases
Subjects
Details
- ISSN :
- 14220067
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a672edc05608d17f4918c438fc71d69b