Back to Search
Start Over
Effects of Brazilian propolis on Leishmania amazonensis
- Source :
- Memórias do Instituto Oswaldo Cruz, Volume: 102, Issue: 2, Pages: 215-220, Published: 23 FEB 2007, Memórias do Instituto Oswaldo Cruz., Vol 102, Iss 2, Pp 215-220 (2007)
- Publication Year :
- 2007
- Publisher :
- Instituto Oswaldo Cruz, Ministério da Saúde, 2007.
-
Abstract
- Leishmaniasis, an endemic parasitosis that leads to chronic cutaneous, mucocutaneous or visceral lesions, is part of those diseases, which still requires improved control tools. Propolis has shown activities against different bacteria, fungi, and parasites. In this study we investigated the effect of four ethanolic extracts of typified propolis collected in different Brazilian states, on Leishmania amazonensis performing assays with promastigote forms, extracellular amastigotes, and on infected peritoneal macrophages. Ethanolic extracts of all propolis samples (BRG, BRPG, BRP-1, and BRV) were capable to reduce parasite load as monitored by the percentage of infected macrophages and the number of intracellular parasites. BRV sample called red propolis, collected in the state of Alagoas, and containing high concentration of prenylated and benzophenones compounds, was the most active extract against L. amazonensis. The anti-Leishmania effect of BRV sample was increased in a concentration and time dependent manner. BRV treatment proved to be non-toxic to macrophage cultures. Since BRV extract at the concentration of 25 microg/ml reduced the parasite load of macrophages while presented no direct toxic to promastigotes and extracellular amastigotes, it was suggested that constituents of propolis intensify the mechanism of macrophage activation leading to killing of L. amazonensis. Our results demonstrate, for the first time, that ethanolic extracts of Brazilian propolis reduce L. amazonensis infection in macrophages, and encourage further studies of this natural compound in animal models of leishmaniasis.
- Subjects :
- Microbiology (medical)
lcsh:Arctic medicine. Tropical medicine
Time Factors
lcsh:RC955-962
natural products
Leishmania mexicana
lcsh:QR1-502
Antiprotozoal Agents
Biology
Parasite load
lcsh:Microbiology
Propolis
Microbiology
Mice
Parasitic Sensitivity Tests
medicine
Extracellular
Macrophage
Animals
Amastigote
leishmaniasis
Cells, Cultured
Mice, Inbred BALB C
Intracellular parasite
Leishmaniasis
medicine.disease
biology.organism_classification
propolis
Macrophages, Peritoneal
Bacteria
Brazil
Leishmania amazonensis
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Memórias do Instituto Oswaldo Cruz, Volume: 102, Issue: 2, Pages: 215-220, Published: 23 FEB 2007, Memórias do Instituto Oswaldo Cruz., Vol 102, Iss 2, Pp 215-220 (2007)
- Accession number :
- edsair.doi.dedup.....a6924fcac13efac408b4d7ff96afc701