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Ykt6-dependent endosomal recycling is required for Wnt secretion in the Drosophila wing epithelium
- Source :
- Development (Cambridge, England), article-version (VoR) Version of Record
- Publication Year :
- 2020
- Publisher :
- The Company of Biologists, 2020.
-
Abstract
- Morphogens are important signalling molecules for tissue development and their secretion requires tight regulation. In the wing imaginal disc of flies, the morphogen Wnt/Wingless is apically presented by the secreting cell and re-internalized before final long-range secretion. Why Wnt molecules undergo these trafficking steps and the nature of the regulatory control within the endosomal compartment remain unclear. Here, we have investigated how Wnts are sorted at the level of endosomes by the versatile v-SNARE Ykt6. Using in vivo genetics, proximity-dependent proteomics and in vitro biochemical analyses, we show that most Ykt6 is present in the cytosol, but can be recruited to de-acidified compartments and recycle Wnts to the plasma membrane via Rab4-positive recycling endosomes. Thus, we propose a molecular mechanism by which producing cells integrate and leverage endocytosis and recycling via Ykt6 to coordinate extracellular Wnt levels.<br />Summary: SNARE-dependent secondary secretion via Rab4 recycling endosomes plays an important role in coordinating long-range extracellular levels of the hydrophobic morphogen Wnt/Wingless.
- Subjects :
- Cell signaling
animal structures
Endosome
Endosomes
Biology
Endocytosis
Epithelium
12. Responsible consumption
Endosomal sorting
R-SNARE Proteins
03 medical and health sciences
0302 clinical medicine
Animals
Drosophila Proteins
Wings, Animal
Compartment (development)
Secretion
Molecular Biology
Wnt signalling
030304 developmental biology
0303 health sciences
Wnt signaling pathway
Morphogen trafficking
Cell biology
Wnt Proteins
Imaginal disc
Drosophila melanogaster
Wnt secretion
030217 neurology & neurosurgery
Research Article
Developmental Biology
Morphogen
Subjects
Details
- ISSN :
- 14779129 and 09501991
- Database :
- OpenAIRE
- Journal :
- Development
- Accession number :
- edsair.doi.dedup.....a69b596948233d89374a84f8537bb4f7