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Analysis of neutral lipids from microalgae by HPLC-ELSD and APCI-MS/MS

Authors :: Caroline Strub
Angélique Fontana
F. Donot
David Egron
Ziya Günata
J. Malinge
Guillaume Cazals
Sabine Schorr-Galindo
Total SA Pôle d'études et de recherche de Lacq (PERL)
TOTAL FINA ELF
Démarche intégrée pour l'obtention d'aliments de qualité (UMR Qualisud)
Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro)
Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)
Institut des Biomolécules Max Mousseron [Pôle Chimie Balard] (IBMM)
Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université de Montpellier (UM)-Ecole Nationale Supérieure de Chimie de Montpellier (ENSCM)
Total New Energy
Senior Scientific Adviser Chemistry
Aquatic Phototrophs Coordinator
Source :: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2013, 942-943, pp.98-106. ⟨10.1016/j.jchromb.2013.10.016⟩
Publication Year :: 2013
Publisher :: HAL CCSD, 2013.
Abstract: International audience; A method was developed to analyze neutral lipids through the use of three triglycerides, four free fatty acids, six di- and four mono-glycerides standards by high performance liquid chromatography (HPLC) normal phase coupled with either with evaporative light scattering detector (ELSD) or with mass spectrometry (MS) operating in atmospheric pressure chemical ionization (APCI) mode. The method was applied to the determination of the neutral lipid fraction from a Botryococcus braunii race A (B. braunii) culture. This method led us to identify neutral lipids synthesized by B. braunii in a single analysis within 45min through HPLC-APCI-MS/MS technique.

Details

Language :: English
ISSN :: 15700232 and 1873376X
Database :: OpenAIRE
Journal :: Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences, Elsevier, 2013, 942-943, pp.98-106. ⟨10.1016/j.jchromb.2013.10.016⟩
Accession number :: edsair.doi.dedup.....a6a2c22c867648e08a225d4a95564d2a
Full Text :: https://doi.org/10.1016/j.jchromb.2013.10.016⟩