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Expression of the TRPM6 in mouse placental trophoblasts; potential role in maternal–fetal calcium transport
- Source :
- The Journal of Physiological Sciences. 67:151-162
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- The placenta is required to transport calcium (Ca2+) from mother to fetus during fetal bone mineralization. In an attempt to clarify the molecular basis of Ca2+ entry for this transport, we identified TRPM6 as a candidate for apical Ca2+ entry pathway. TRPM6 mRNA increased during the last 4 days of pregnancy, coinciding with fetal bone mineralization in mice. TRPM6 mRNA and protein was localized in the trophoblasts in labyrinth where the maternal–fetal Ca2+ transport occurs. In patch-clamp recordings, we observed TRPM6/TRPM7-like currents in mouse trophoblasts after starting fetal bone mineralization but not before mineralization. Plasma membrane Ca2+ permeability was significantly increased in TRPM6/TRPM7 expressed HEK293 cells under physiological Mg2+ and ATP concentration but not in TRPM6 or TRPM7 homomer-expressing cells. These results suggest that TRPM6 is functionally expressed in mouse placental trophoblasts, implicating in maternal–fetal Ca2+ transport likely with TRPM7, which might enable to sustain fetal bone mineralization.
- Subjects :
- 0301 basic medicine
Cell physiology
medicine.medical_specialty
Cell Membrane Permeability
Physiology
Placenta
TRPM Cation Channels
chemistry.chemical_element
Calcium
Mice
03 medical and health sciences
0302 clinical medicine
Pregnancy
TRPM7
Internal medicine
medicine
Animals
Humans
Maternal-Fetal Exchange
Ion transporter
Messenger RNA
Fetus
Ion Transport
Chemistry
HEK 293 cells
Trophoblasts
Cell biology
HEK293 Cells
030104 developmental biology
Endocrinology
medicine.anatomical_structure
embryonic structures
Female
030217 neurology & neurosurgery
Subjects
Details
- ISSN :
- 18806562 and 18806546
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- The Journal of Physiological Sciences
- Accession number :
- edsair.doi.dedup.....a6c0efe06050c0b574e819e37e69d1d4
- Full Text :
- https://doi.org/10.1007/s12576-016-0449-0