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Identification of the Immunogenic Outer Membrane Protein A Antigen of Haemophilus parasuis by a Proteomics Approach and Passive Immunization with Monoclonal Antibodies in Mice ▿
- Publication Year :
- 2011
- Publisher :
- American Society for Microbiology, 2011.
-
Abstract
- Monoclonal antibodies (MAbs) againstHaemophilus parasuiswere generated by fusing spleen cells from BALB/c mice immunized with whole bacterial cells with SP2/0 murine myeloma cells. Desirable hybridomas were screened by enzyme-linked immunosorbent assay (ELISA). Neutralizing MAb 1D8 was selected in protection assays. ELISA results demonstrated that 1D8 can react with all 15 serotypes ofH. parasuisand field isolateH. parasuisHLJ-018. Passive immunization studies showed that mice inoculated intraperitoneally with 1D8 had significantly reduced prevalence ofH. parasuiscolonization in the blood, lung, spleen, and liver and had prolonged survival time compared to that of the control group. Furthermore, the passive transfer experiment indicated that MAb 1D8 can protect mice from both homologous and heterologous challenges withH. parasuis. Using two-dimensional gel electrophoresis (2-DE), the immunoreactive protein target for MAb 1D8 was identified. The data presented confirm the protective role of MAb 1D8 and identify OmpA as the target of the protective monoclonal antibody. The data suggest that OmpA is a promising candidate for a subunit vaccine againstH. parasuis.
- Subjects :
- Microbiology (medical)
Serotype
Proteomics
Haemophilus Infections
medicine.drug_class
Clinical Biochemistry
Immunology
Heterologous
Spleen
Enzyme-Linked Immunosorbent Assay
Monoclonal antibody
Veterinary Immunology
Haemophilus parasuis
Mice
Antigen
Haemophilus
medicine
Immunology and Allergy
Animals
Electrophoresis, Gel, Two-Dimensional
Antigens, Bacterial
Mice, Inbred BALB C
biology
Immunization, Passive
Animal Structures
Antibodies, Monoclonal
biology.organism_classification
Virology
Antibodies, Bacterial
Antibodies, Neutralizing
Survival Analysis
Bacterial Load
medicine.anatomical_structure
Immunization
biology.protein
Antibody
Bacterial Outer Membrane Proteins
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a6c979a15e4dcca2467b5d669952c44a