Back to Search
Start Over
Characterization of the recombinant Candida albicans β-1,2-mannosyltransferase that initiates the β-mannosylation of cell wall phosphopeptidomannan
- Source :
- Biochemical Journal, Biochemical Journal, 2013, 457 (2), pp.347-360. ⟨10.1042/BJ20131012⟩, Biochemical Journal, Portland Press, 2013, 457 (2), pp.347-360. ⟨10.1042/BJ20131012⟩
- Publication Year :
- 2013
- Publisher :
- Portland Press Ltd., 2013.
-
Abstract
- The presence of β-mannosides in their cell walls confers specific features on the pathogenic yeasts Candida albicans and Candida glabrata compared with non-pathogenic yeasts. In the present study, we investigated the enzymatic properties of Bmt1 (β-mannosyltransferase 1), a member of the recently identified β-mannosyltransferase family, from C. albicans. A recombinant soluble enzyme lacking the N-terminal region was expressed as a secreted protein from the methylotrophic yeast Pichia pastoris. In parallel, functionalized natural oligosaccharides isolated from Saccharomyces cerevisiae and a C. albicans mutant strain, as well as synthetic α-oligomannosides, were prepared and used as potential acceptor substrates. Bmt1p preferentially utilizes substrates containing linear chains of α-1,2-linked mannotriose or mannotetraose. The recombinant enzyme consecuti-vely transfers two mannosyl units on to these acceptors, leading to the production of α-mannosidase-resistant oligomannosides. NMR experiments further confirmed the presence of a terminal βMan (β-1,2-linked mannose) unit in the first enzyme product. In the future, a better understanding of specific β-1,2-mannosyltransferase molecular requirements will help the design of new potential antifungal drugs.
- Subjects :
- Phosphopeptides
Mannosyltransferase
Mannose
Biology
Mannosyltransferases
Biochemistry
Pichia pastoris
Microbiology
Mannans
03 medical and health sciences
chemistry.chemical_compound
Cell Wall
Candida albicans
Molecular Biology
030304 developmental biology
0303 health sciences
[CHIM.ORGA]Chemical Sciences/Organic chemistry
030302 biochemistry & molecular biology
Cell Biology
biology.organism_classification
Recombinant Proteins
Corpus albicans
Yeast
chemistry
Mannosylation
Subjects
Details
- ISSN :
- 14708728 and 02646021
- Volume :
- 457
- Database :
- OpenAIRE
- Journal :
- Biochemical Journal
- Accession number :
- edsair.doi.dedup.....a6d6d9b7fa03ecf469cb9c8bb125d32d
- Full Text :
- https://doi.org/10.1042/bj20131012