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Molecular detection of Enterocytozoon bieneusi in alpacas (Vicugna pacos) in Xinjiang, China
- Source :
- Parasite, Vol 26, p 31 (2019), Parasite
- Publication Year :
- 2019
- Publisher :
- EDP Sciences, 2019.
-
Abstract
- Enterocytozoon bieneusi, an obligate intracellular pathogen, can infect a wide variety of hosts. This study aimed to determine the prevalence and molecular characteristics of E. bieneusi in alpacas (Vicugna pacos) in China. A total of 185 alpaca fecal samples were collected from five herds in Tacheng, Wensu, Hejing, Qinghe, and Nilka counties in Xinjiang Uygur Autonomous Region. Enterocytozoon bieneusi was detected by nested PCR of the internal transcribed spacer (ITS) region. Twenty-eight fecal samples (15.1%, 28/185) were positive for E. bieneusi, with the highest prevalence in alpacas from Qinghe (42.9%, 15/35). Four E. bieneusi genotypes were identified, which included two known (P and ALP3) and two novel (ALP7 and ALP8) genotypes. Genotype ALP3 was the dominant genotype (57.1%, 16/28), followed by genotypes P (32.1%, 9/28), ALP7 (7.1%, 2/28), and ALP8 (2.6%, 1/28). Phylogenetic analysis revealed that three genotypes (P, ALP7, and ALP3) clustered into group 1, whereas genotype ALP8 clustered into group 8. This is the first report of E. bieneusi infection and genetic diversity in alpacas from Xinjiang, China.
- Subjects :
- 0301 basic medicine
China
Veterinary medicine
Short Note
genotype
Veterinary (miscellaneous)
030231 tropical medicine
Vicugna pacos
Polymerase Chain Reaction
lcsh:Infectious and parasitic diseases
Feces
03 medical and health sciences
fluids and secretions
0302 clinical medicine
DNA, Ribosomal Spacer
Microsporidiosis
parasitic diseases
Genotype
Prevalence
E. bieneusi
biology.domesticated_animal
Animals
lcsh:RC109-216
Enterocytozoon bieneusi
Internal transcribed spacer
DNA, Fungal
zoonotic
Phylogeny
alpacas
biology
fungi
Fungal genetics
Genetic Variation
Sequence Analysis, DNA
Enterocytozoon
biology.organism_classification
030104 developmental biology
Infectious Diseases
Insect Science
Multilocus sequence typing
Animal Science and Zoology
Parasitology
ITS
Camelids, New World
Nested polymerase chain reaction
Multilocus Sequence Typing
Subjects
Details
- ISSN :
- 17761042
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Parasite
- Accession number :
- edsair.doi.dedup.....a6e1b74ca0260708460c23caf9f868f8
- Full Text :
- https://doi.org/10.1051/parasite/2019031