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Waves of calcium depletion in the sarcoplasmic reticulum of vascular smooth muscle cells: an inside view of spatiotemporal Ca2+ regulation
- Source :
- PLoS ONE, Vol 8, Iss 2, p e55333 (2013), PLoS ONE
- Publication Year :
- 2013
- Publisher :
- Public Library of Science (PLoS), 2013.
-
Abstract
- Agonist-stimulated smooth muscle Ca2+ waves regulate blood vessel tone and vasomotion. Previous studies employing cytoplasmic Ca2+ indicators revealed that these Ca2+ waves were stimulated by a combination of inositol 1,4,5-trisphosphate- and Ca2+ -induced Ca2+ release from the endo/sarcoplasmic reticulum. Herein, we present the first report of endothelin-1 stimulated waves of Ca2+ depletion from the sarcoplasmic reticulum of vascular smooth muscle cells using a calsequestrin-targeted Ca2+ indicator. Our findings confirm that these waves are due to regenerative Ca2+ -induced Ca2+ release by the receptors for inositol 1,4,5-trisphosphate. Our main new finding is a transient elevation in SR luminal Ca2+ concentration ([Ca2+](SR)) both at the site of wave initiation, just before regenerative Ca2+ release commences, and at the advancing wave front, during propagation. This strongly suggests a role for [Ca2+](SR) in the activation of inositol 1,4,5-trisphosphate receptors during agonist-induced calcium waves. In addition, quantitative analysis of the gradual decrease in the velocity of the depletion wave, observed in the absence of external Ca2+, indicates continuity of the lumen of the sarcoplasmic reticulum network. Finally, our observation that the depletion wave was arrested by the nuclear envelope may have implications for selective Ca2+ signalling.
- Subjects :
- Vascular smooth muscle
Anatomy and Physiology
lcsh:Medicine
Vasomotion
Cardiovascular
Muscle, Smooth, Vascular
chemistry.chemical_compound
0302 clinical medicine
Molecular Cell Biology
Signaling in Cellular Processes
Inositol
Receptor
lcsh:Science
Musculoskeletal System
Cells, Cultured
Calcium signaling
0303 health sciences
Multidisciplinary
Endothelin-1
Muscle Biochemistry
Signaling Cascades
Sarcoplasmic Reticulum
Calibration
Thapsigargin
Muscle
Medicine
Cellular Types
Research Article
Signal Transduction
medicine.medical_specialty
chemistry.chemical_element
Calcium
Muscle Types
03 medical and health sciences
Vascular Biology
Internal medicine
medicine
Animals
Calcium Signaling
Biology
030304 developmental biology
Muscle Cells
Endoplasmic reticulum
lcsh:R
Rats
Endocrinology
chemistry
Calcium Signaling Cascade
Biophysics
lcsh:Q
030217 neurology & neurosurgery
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 8
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- PLoS ONE
- Accession number :
- edsair.doi.dedup.....a6e6450be45a3eb87f7b551ab1496b6c