Back to Search
Start Over
Uniform aligned bioconjugation of biomolecule motifs for integration within microfabricated microfluidic devices
- Source :
- Analytical Biochemistry. (2):195-205
- Publisher :
- Elsevier Inc.
-
Abstract
- Full details and a step-by-step guide suitable for printing proteins aligned to micron-sized sensors and subsequent integration and alignment of microfluidic structures are presented. The precise alignment and grafting of micron-sized biomolecule patterns with an underlying substrate at predefined locations is achieved using a novel semi-automated microcontact printer. Through integration of optical alignment methods in the x, y, and z directions, uniform contact of micron-sized stamps is achieved. Feature compression of the stamp is avoided by fine control of the stamp during contact. This printing method has been developed in combination with robust, compatible bioconjugate chemistry for patterning of a dextran-functionalized silicon oxide substrate with a NeutrAvidin-“inked” stamp and subsequent incubation with a biotin-functionalized protein. The bioconjugate chemistry is such that uniform coverage of the protein (without denaturation) over the printed motif is obtained and reproduction of the initial mask shape and dimensions is achieved. Later integration with a microfluidic structure aligned with the printed motif on the substrate is also described.
- Subjects :
- Optical alignment
Microcontact printing
Microfluidics
Biophysics
Biotin
Nanotechnology
Bioaffinity sensors
Biosensing Techniques
Biochemistry
Antibodies
law.invention
law
Lab-On-A-Chip Devices
Humans
Molecular Biology
chemistry.chemical_classification
Bioconjugation
Lab-on-a-chip
Tumor Necrosis Factor-alpha
Photoelectron Spectroscopy
Biomolecule
Dextrans
Cell Biology
Carbocyanines
Microfluidic Analytical Techniques
Avidin
Silicon Dioxide
Biosensors
chemistry
Microfluidic
Cytokines
Microtechnology
Microfabrication
Biosensor
Subjects
Details
- Language :
- English
- ISSN :
- 00032697
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Analytical Biochemistry
- Accession number :
- edsair.doi.dedup.....a7123a5d1a0ca8b035b2d6c215ee78b5
- Full Text :
- https://doi.org/10.1016/j.ab.2012.02.020