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Protective Effect of Low Molecular Weight Seleno-Aminopolysaccharide on the Intestinal Mucosal Oxidative Damage
- Source :
- Marine Drugs, Volume 17, Issue 1, Marine Drugs, Vol 17, Iss 1, p 64 (2019)
- Publication Year :
- 2019
- Publisher :
- Multidisciplinary Digital Publishing Institute, 2019.
-
Abstract
- Low molecular weight seleno-aminopolysaccharide (LSA) is an organic selenium compound comprising selenium and low molecular weight aminopolysaccharide (LA), a low molecular weight natural linear polysaccharide derived from chitosan. LSA has been found to exert strong pharmacological activity. In this study, we aimed to investigate the protective effect of LSA on intestinal mucosal oxidative stress in a weaning piglet model by detecting the growth performance, intestinal mucosal structure, antioxidant indices, and expression level of intracellular transcription factor nuclear factor erythroid 2-related factor 2 (Nrf2) and its related factors. Our results indicated that LSA significantly increased the average daily gain and feed/gain (p &lt<br />0.05), suggesting that LSA can effectively promote the growth of weaning piglets. The results of scanning electron microscope (SEM) microscopy showed that LSA effectively reduced intestinal damage, indicating that LSA improved the intestinal stress response and protected the intestinal structure integrity. In addition, diamine oxidase (DAO) and d-lactic acid (d-LA) levels remarkably decreased in LSA group compared with control group (p &lt<br />0.05), suggesting that LSA alleviated the damage and permeability of weaning piglets. LSA significantly increased superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC) levels, but decreased malondialdehyde (MDA) level, indicating that LSA significantly enhanced the antioxidant capacity and reduced oxidative stress in weaning piglets. RT-PCR results showed that LSA significantly increased GSH-Px1, GSH-Px2, SOD-1, SOD-2, CAT, Nrf2, HO-1, and NQO1 gene expression (p &lt<br />0.05). Western blot analysis revealed that LSA activated the Nrf2 signaling pathway by downregulating the expression of Keap1 and upregulating the expression of Nrf2 to protect intestinal mucosa against oxidative stress. Collectively, LSA reduced intestinal mucosal damage induced by oxidative stress via Nrf2-Keap1 pathway in weaning stress of infants.
- Subjects :
- Male
Antioxidant
medicine.medical_treatment
Sus scrofa
Pharmaceutical Science
oxidative damage
medicine.disease_cause
chemistry.chemical_compound
0302 clinical medicine
Intestinal mucosa
Drug Discovery
lcsh:QH301-705.5
Pharmacology, Toxicology and Pharmaceutics (miscellaneous)
chemistry.chemical_classification
0303 health sciences
Kelch-Like ECH-Associated Protein 1
biology
Chemistry
Glutathione peroxidase
Malondialdehyde
Animals, Suckling
Treatment Outcome
Catalase
030211 gastroenterology & hepatology
Diamine oxidase
Signal Transduction
NF-E2-Related Factor 2
Weaning
Article
Superoxide dismutase
Andrology
Selenium
03 medical and health sciences
Stress, Physiological
medicine
Animals
Humans
low molecular seleno-aminopolysaccharide
030304 developmental biology
Chitosan
Infant
Molecular Weight
Disease Models, Animal
Oxidative Stress
intestinal mucosa
Nrf2–Keap1 pathway
lcsh:Biology (General)
Diarrhea, Infantile
Dietary Supplements
Microscopy, Electron, Scanning
biology.protein
Oxidative stress
Subjects
Details
- Language :
- English
- ISSN :
- 16603397
- Database :
- OpenAIRE
- Journal :
- Marine Drugs
- Accession number :
- edsair.doi.dedup.....a715216adc6c91c5ef4ce44a95c71a1f
- Full Text :
- https://doi.org/10.3390/md17010064