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The proliferation role of LH on porcine primordial germ cell‐like cells (pPGCLCs) through ceRNA network construction
- Source :
- Clinical and Translational Medicine, Vol 11, Iss 10, Pp n/a-n/a (2021), Clinical and Translational Medicine
- Publication Year :
- 2021
- Publisher :
- Wiley, 2021.
-
Abstract
- Background The transdifferentiation of skin‐derived stem cells (SDSCs) into primordial germ cell‐like cells (PGCLCs) is one of the major breakthroughs in the field of stem cells research in recent years. This technology provides a new theoretical basis for the treatment of human infertility. However, the transdifferentiation efficiency of SDSCs to PGCLCs is very low, and scientists are still exploring ways to improve this efficiency or promote the proliferation of PGCLCs. This study aims to investigate the molecular mechanism of luteinising hormone (LH) to enhance porcine PGCLCs (pPGCLCs) proliferation. Results In this study, we dissected the proliferation regulatory network of pPGCLCs by whole transcriptome sequencing, and the results showed that the pituitary‐secreted reproductive hormone LH significantly promoted the proliferation of pPGCLCs. We combined whole transcriptome sequencing and related validation experiments to explore the mechanism of LH on the proliferation of pPGCLCs, and found that LH could affect the expression of Hippo signalling pathway‐related mRNAs, miRNAs and lncRNAs in pPGCLCs. Conclusions For the first time, we found that LH promotes pPGCLCs proliferation through the competing endogenous RNA (ceRNA) regulatory networks and Hippo signalling pathway. This finding may help to elucidate the molecular mechanism by which LH promotes pPGCLCs proliferation.<br />LH promotes the proliferation of pPGCLCs in vitro.LH‐stimulated proliferation is accompanied by decreased apoptosis.Hippo signalling pathway‐associated ceRNA networks played an important role in the proliferation of pPGCLCs.
- Subjects :
- LH
ceRNA network
Medicine (General)
Swine
proliferation
Medicine (miscellaneous)
Biology
R5-920
microRNA
Animals
Hippo Signaling Pathway
Research Articles
Hippo signalling pathway
Cell Proliferation
pPGCLCs
Competing endogenous RNA
Mechanism (biology)
Stem Cells
Transdifferentiation
Luteinizing Hormone
Hedgehog signaling pathway
Cell biology
Signalling
Germ Cells
Molecular Medicine
RNA, Long Noncoding
Stem cell
Transcriptome
Hormone
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 20011326
- Volume :
- 11
- Issue :
- 10
- Database :
- OpenAIRE
- Journal :
- Clinical and Translational Medicine
- Accession number :
- edsair.doi.dedup.....a738b87f21501f7a167bc5df750393d8