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Characterization of steroidal glycosides from the extract of Paris Polyphylla var. Yunnanensis by UPLC/Q-TOF MSE
- Source :
- Journal of Pharmaceutical and Biomedical Analysis. 62:235-249
- Publication Year :
- 2012
- Publisher :
- Elsevier BV, 2012.
-
Abstract
- Steroidal saponins in Rhizoma Paridis attract scientific attentions for their structural diversity and significant bioactivities. In this work, an ultra performance liquid chromatography coupled with a hybrid quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF MS) was used to rapidly separate and identify steroidal saponins from the extract of the rhizome of Paris polyphylla var. yunnanensis (PPY). The fragment ions from glycosidic and cross-ring cleavages offered a wealth of structural information that is indicative to the aglycones, sugar types and the connecting sequence of sugar units. Based on the exact mass information, the fragmentation characteristics, and the LC retention times of 21 reference steroidal saponin standards, 98 constituents were tentatively identified with their structures proposed, which covered more than 30 types of steroidal aglycones. The 98 constituents consist of 22 pairs of structural isomers, and 40 steroidal glycosides that are identified for the first time from the nature.
- Subjects :
- Spectrometry, Mass, Electrospray Ionization
Molecular Sequence Data
Clinical Biochemistry
Saponin
Pharmaceutical Science
Mass spectrometry
High-performance liquid chromatography
Analytical Chemistry
Magnoliopsida
Drug Discovery
Structural isomer
Glycosides
Sugar
Spectroscopy
chemistry.chemical_classification
Chromatography
biology
Plant Extracts
Chemistry
Paris polyphylla
Glycosidic bond
biology.organism_classification
Rhizome
Carbohydrate Sequence
Steroids
Chromatography, Liquid
Subjects
Details
- ISSN :
- 07317085
- Volume :
- 62
- Database :
- OpenAIRE
- Journal :
- Journal of Pharmaceutical and Biomedical Analysis
- Accession number :
- edsair.doi.dedup.....a7567ef5388d3bc79bc4e04f87160435