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Secreted Proteases Control Autolysin-mediated Biofilm Growth of Staphylococcus aureus*
- Source :
- The Journal of Biological Chemistry
- Publication Year :
- 2013
- Publisher :
- American Society for Biochemistry and Molecular Biology, 2013.
-
Abstract
- Background: Esp, a secreted protease of Staphylococcus epidermidis, blocks biofilm formation of Staphylococcus aureus and its ability to colonize human nares. Results: Esp cleaves autolysin, thereby preventing the release of staphylococcal DNA as biofilm matrix. Conclusion: Secreted proteases control S. aureus biofilm development and host colonization. Significance: Methods that promote autolysin degradation may also prevent S. aureus colonization of humans.<br />Staphylococcus epidermidis, a commensal of humans, secretes Esp protease to prevent Staphylococcus aureus biofilm formation and colonization. Blocking S. aureus colonization may reduce the incidence of invasive infectious diseases; however, the mechanism whereby Esp disrupts biofilms is unknown. We show here that Esp cleaves autolysin (Atl)-derived murein hydrolases and prevents staphylococcal release of DNA, which serves as extracellular matrix in biofilms. The three-dimensional structure of Esp was revealed by x-ray crystallography and shown to be highly similar to that of S. aureus V8 (SspA). Both atl and sspA are necessary for biofilm formation, and purified SspA cleaves Atl-derived murein hydrolases. Thus, S. aureus biofilms are formed via the controlled secretion and proteolysis of autolysin, and this developmental program appears to be perturbed by the Esp protease of S. epidermidis.
- Subjects :
- DNA, Bacterial
Models, Molecular
Proteases
Staphylococcus aureus
Staphylococcal infections
medicine.disease_cause
Crystallography, X-Ray
Biochemistry
Microbiology
Protein Structure, Secondary
Substrate Specificity
03 medical and health sciences
fluids and secretions
Bacterial Proteins
Staphylococcus epidermidis
Antibiosis
medicine
Humans
N-acetylmuramoyl-L-alanine amidase
Molecular Biology
030304 developmental biology
0303 health sciences
biology
030306 microbiology
Biofilm
fungi
Autolysin
Biofilm matrix
Cell Biology
N-Acetylmuramoyl-L-alanine Amidase
biochemical phenomena, metabolism, and nutrition
Staphylococcal Infections
medicine.disease
biology.organism_classification
3. Good health
Extracellular Matrix
Protein Structure, Tertiary
Biofilms
Bacterial Pathogenesis
Electrophoresis, Polyacrylamide Gel
Serine Protease
Nasal Cavity
Serine Proteases
Subjects
Details
- Language :
- English
- ISSN :
- 1083351X and 00219258
- Volume :
- 288
- Issue :
- 41
- Database :
- OpenAIRE
- Journal :
- The Journal of Biological Chemistry
- Accession number :
- edsair.doi.dedup.....a772f6c872aabf4315a53e8a0b337874