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A Confirmed Case of SARS-CoV-2 Pneumonia With Negative Routine Reverse Transcriptase-Polymerase Chain Reaction and Virus Variation in Guangzhou, China
- Source :
- Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America, Clinical Infectious Diseases
- Publication Year :
- 2020
-
Abstract
- Background Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pneumonia is a newly recognized disease, and its diagnosis is primarily confirmed by routine reverse transcriptase -polymerase chain reaction (RT-PCR) detection of SARS-CoV-2. Methods However, we report a confirmed case of SARS-CoV-2 pneumonia with a negative routine RT-PCR. Results This case was finally diagnosed by nanopore sequencing combined with antibody of SARS-CoV-2. Simultaneously, the ORF and NP gene variations of SARS-CoV-2 were found. Conclusions This case highlighted that false-negative results could be present in routine RT-PCR diagnosis, especially with virus variation. Currently, nanopore pathogen sequencing and antibody detection have been found to be effective in clinical diagnosis.
- Subjects :
- 0301 basic medicine
Microbiology (medical)
China
Nanopore sequencing
viruses
Virus variation
Virus
law.invention
03 medical and health sciences
0302 clinical medicine
law
Major Article
medicine
diagnostics
Humans
030212 general & internal medicine
skin and connective tissue diseases
Pathogen
Polymerase chain reaction
metagenomics
biology
business.industry
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
RNA-Directed DNA Polymerase
fungi
virus diseases
COVID-19
Pneumonia
golden standard
medicine.disease
Virology
Reverse transcriptase
respiratory tract diseases
Editorial Commentary
030104 developmental biology
Infectious Diseases
AcademicSubjects/MED00290
consensus
biology.protein
Antibody
business
Routine RT-PCR
case definition
Subjects
Details
- ISSN :
- 15376591
- Volume :
- 73
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
- Accession number :
- edsair.doi.dedup.....a7970471e3a8de5c08c3ae8e2966e247