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Plasma Concentrations and Subcutaneous Adipose Tissue mRNA Expression of Clusterin in Obesity and Type 2 Diabetes Mellitus: the Effect of Short-Term Hyperinsulinemia, Very-Low-Calorie Diet and Bariatric Surgery
- Source :
- ResearcherID, Scopus-Elsevier
-
Abstract
- Clusterin is a heterodimeric glycoprotein with wide range of functions. To further explore its possible regulatory role in energy homeostasis and in adipose tissue, we measured plasma clusterin and its mRNA expression in subcutaneous adipose tissue (SCAT) of 15 healthy lean women, 15 obese women (OB) and 15 obese women with type 2 diabetes mellitus (T2DM) who underwent a 2-week very low-calorie diet (VLCD), 10 obese women without T2DM who underwent laparoscopic sleeve gastrectomy (LSG) and 8 patients with T2DM, 8 patients with impaired glucose tolerance (IGT) and 8 normoglycemic patients who underwent hyperinsulinemic euglycemic clamp (HEC). VLCD decreased plasma clusterin in OB but not in T2DM patients while LSG and HEC had no effect. Clusterin mRNA expression in SCAT at baseline was increased in OB and T2DM patients compared with controls. Clusterin mRNA expression decreased 6 months after LSG and remained decreased 12 months after LSG. mRNA expression of clusterin was elevated at the end of HEC compared with baseline only in normoglycemic but not in IGT or T2DM patients. In summary, our data suggest a possible local regulatory role for clusterin in the adipose tissue rather than its systemic involvement in the regulation of energy homeostasis.
- Subjects :
- 0301 basic medicine
medicine.medical_specialty
endocrine system diseases
Physiology
food.diet
Protein Array Analysis
Subcutaneous Fat
Adipose tissue
Bariatric Surgery
Energy homeostasis
Impaired glucose tolerance
03 medical and health sciences
0302 clinical medicine
food
Diabetes mellitus
Internal medicine
medicine
Hyperinsulinemia
Humans
Obesity
RNA, Messenger
Caloric Restriction
Clusterin
biology
business.industry
nutritional and metabolic diseases
Type 2 Diabetes Mellitus
General Medicine
Middle Aged
medicine.disease
eye diseases
Surgery
Very low calorie diet
030104 developmental biology
Endocrinology
Diabetes Mellitus, Type 2
Case-Control Studies
biology.protein
Female
sense organs
business
Energy Metabolism
030217 neurology & neurosurgery
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- ResearcherID, Scopus-Elsevier
- Accession number :
- edsair.doi.dedup.....a7976ff1c90f6e9194f9e4b058a4b3e6