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A link between planar polarity and staircase-like bundle architecture in hair cells
- Publication Year :
- 2016
- Publisher :
- The Company of Biologists Ltd, 2016.
-
Abstract
- Sensory perception in the inner ear relies on the hair bundle, the highly polarized brush of movement detectors crowning hair cells. We previously showed that, in the mouse cochlea, the edge of the forming bundle is defined by the 'bare zone', a microvilli-free sub-region of apical membrane specified by the mInsc-LGN-Gαi protein complex. We now report that LGN and Gαi also occupy the very tip of stereocilia that directly abut the bare zone. We demonstrate that LGN and Gαi are both essential to promote the elongation and differential identity of stereocilia across rows. Interestingly, we also uncover that total LGN-Gαi protein amounts are actively balanced between the bare zone and stereocilia tips, suggesting that early planar asymmetry of protein enrichment at the bare zone confers adjacent stereocilia their tallest identity. We propose that LGN and Gαi participate in a long-inferred signal originating outside of the bundle to model its staircase-like architecture, a property essential for direction-sensitivity to mechanical deflection and hearing.
- Subjects :
- 0301 basic medicine
Research Report
genetic structures
Mouse Cochlea
Cell Cycle Proteins
Mice, Transgenic
Biology
Deafness
GTP-Binding Protein alpha Subunits, Gi-Go
03 medical and health sciences
Mice
Planar
Hair Cells, Auditory
medicine
otorhinolaryngologic diseases
Animals
Inner ear
Molecular Biology
Body Patterning
Cell Polarity
Gene Expression Regulation, Developmental
Anatomy
Apical membrane
Embryo, Mammalian
Protein enrichment
Cochlea
Mice, Inbred C57BL
030104 developmental biology
medicine.anatomical_structure
Bundle
Biophysics
Hair cell
sense organs
Elongation
Carrier Proteins
Developmental Biology
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a79fd3c3c3b139d5f68b0a484baf993c