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IFN-stimulated Gene Expression Is a Useful Potential Molecular Marker of Response to Antiviral Treatment with Peg-IFNα 2b and Ribavirin in Patients with Hepatitis C Virus Genotype 1
- Source :
- Archives of Medical Research. 42:28-33
- Publication Year :
- 2011
- Publisher :
- Elsevier BV, 2011.
-
Abstract
- Background and Aims We undertook this study to determine the baseline gene expression of IFI27, IFIT1, IFI6, ISG15, IRF-1, IRF-3, OAS-2 and CXCL10 and its usefulness as molecular markers of response to antiviral treatment with peg-IFNα 2b/RBV in patients with hepatitis C virus genotype 1 (HCV-1). Methods Gene expression was analyzed by RT-PCR in baseline liver biopsies from 42 HCV-1 patients who were treated with Peg-IFNα 2b/RBV for 48 weeks. In addition, we investigated gene expression of these genes in a second liver biopsy obtained 24 weeks post-treatment in sustained viral response (SVR) and relapser patients. Results Thirteen patients achieved SVR, four were relapsers, four patients with viral response (VR) discontinued the following for 24 weeks post-treatment and 21 patients did not respond to antiviral therapy (NR). All patients with HCV-1 showed gene overexpression in baseline liver tissue, but only IFI27, IFIT1, IFI6, ISG15, and CXCL10 showed differential gene expression, which is inversely related to the response to antiviral therapy. Thus, liver tissue of NR patients showed upregulation of these genes, whereas patients with SVR gene expression level was significantly lower. Furthermore, 24 weeks afterwards treatment, SVR patients showed a significant downregulation of such genes, which was consistent with the RNA-HCV suppression. ISGs (IFI27, IFIT1, IFI6) and chemokine CXCL10 showed the best positive and negative predictive values on SVR to IFN/RBV therapy (range: 70.8–75% and 71.43–82.35%), respectively. Conclusions IFI27, IFIT1, IFI6, ISG15, and CXCL10 genes are potential biological markers useful for predicting response to Peg-IFNα 2b/RBV therapy in HCV-1 patients.
- Subjects :
- Adult
Male
Hepatitis C virus
Gene Expression
Hepacivirus
Interferon alpha-2
Biology
medicine.disease_cause
Antiviral Agents
Biomarkers, Pharmacological
Polyethylene Glycols
chemistry.chemical_compound
Pharmacotherapy
Ribavirin
Gene expression
medicine
Humans
CXCL10
Aged
medicine.diagnostic_test
Interferon-alpha
virus diseases
General Medicine
Hepatitis C
Hepatitis C, Chronic
Middle Aged
medicine.disease
ISG15
Recombinant Proteins
Treatment Outcome
chemistry
Liver biopsy
Immunology
Drug Therapy, Combination
Female
Interferons
Subjects
Details
- ISSN :
- 01884409
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Archives of Medical Research
- Accession number :
- edsair.doi.dedup.....a7aa10e43fe6b419829c75171b0cecaa