Back to Search
Start Over
Kidney intercalated cells are phagocytic and acidify internalized uropathogenic Escherichia coli
- Source :
- Nature Communications, Vol 12, Iss 1, Pp 1-15 (2021), Nature Communications
- Publication Year :
- 2021
- Publisher :
- Nature Portfolio, 2021.
-
Abstract
- Kidney intercalated cells are involved in acid-base homeostasis via vacuolar ATPase expression. Here we report six human intercalated cell subtypes, including hybrid principal-intercalated cells identified from single cell transcriptomics. Phagosome maturation is a biological process that increases in biological pathway analysis rank following exposure to uropathogenic Escherichia coli in two of the intercalated cell subtypes. Real time confocal microscopy visualization of murine renal tubules perfused with green fluorescent protein expressing Escherichia coli or pHrodo Green E. coli BioParticles demonstrates that intercalated cells actively phagocytose bacteria then acidify phagolysosomes. Additionally, intercalated cells have increased vacuolar ATPase expression following in vivo experimental UTI. Taken together, intercalated cells exhibit a transcriptional response conducive to the kidney’s defense, engulf bacteria and acidify the internalized bacteria. Intercalated cells represent an epithelial cell with characteristics of professional phagocytes like macrophages.<br />Kidney intercalated cells are involved in acid-base homeostasis in the kidneys. Here, the authors use single cell transcriptomics and find that interalated cells exhibit a transcriptional response conducive to defense and can engulf and acidify internalized bacteria, similarly to professional phagocytes.
- Subjects :
- 0301 basic medicine
Cell biology
Vacuolar Proton-Translocating ATPases
Phagocytosis
Science
education
030232 urology & nephrology
General Physics and Astronomy
Mice, Transgenic
medicine.disease_cause
Kidney
General Biochemistry, Genetics and Molecular Biology
Article
Green fluorescent protein
Biological pathway
03 medical and health sciences
0302 clinical medicine
Phagosome maturation
medicine
Animals
Humans
Uropathogenic Escherichia coli
Intercalated Cell
Kidney Tubules, Collecting
Escherichia coli
Cells, Cultured
health care economics and organizations
Phagocytes
Multidisciplinary
Microscopy, Confocal
biology
Chemistry
Gene Expression Profiling
Epithelial Cells
General Chemistry
biology.organism_classification
Mice, Inbred C57BL
030104 developmental biology
Mechanisms of disease
Single-Cell Analysis
Acids
Homeostasis
Bacteria
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 12
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....a7c8fb7d6a8b2354c40d1c2608ab41e4