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Identification Of Major Adh Genes In Ethanol Metabolism Of Pichia Pastoris
Identification Of Major Adh Genes In Ethanol Metabolism Of Pichia Pastoris
- Publication Year :
- 2020
- Publisher :
- Aperta, 2020.
-
Abstract
- The methylotrophic yeast Pichia pastoris (syn. Komagataella phaffii) is a successful host widely used in recombinant protein production. The widespread use of a methanol-regulated alcohol oxidase 1 (AOX1) promoter for recombinant protein production has directed studies particularly about methanol metabolism in this yeast. Although there is comprehensive knowledge about methanol metabolism, there are other mechanisms in P. pastoris that have not been investigated yet, such as ethanol metabolism. The gene responsible for the consumption of ethanol ADH2 (XM_002491337, known as ADH3) was identified and characterized in our previous study. In this study, the ADH genes (XM_002489969, XM_002491163, XM_002493969) in P. pastoris genome were investigated to determine their roles in ethanol production by gene disruption analysis. We report that the ADH900 (XM_002491163) is the main gene responsible for ethanol production in P. pastoris. The ADH2 gene, previously identified as the only gene responsible for ethanol consumption, also plays a minor role in ethanol production in the absence of the ADH900 gene. The investigation of the carbon source regulation mechanism has also revealed that the ADH2 gene exhibit similar expression behaviours with ADH900 on glucose, glycerol, and methanol, however, it is strongly induced by ethanol.
- Subjects :
- 0106 biological sciences
Glycerol
crabtree-negative yeast
Genes, Fungal
Bioengineering
Biology
01 natural sciences
Applied Microbiology and Biotechnology
Biochemistry
Pichia
Pichia pastoris
ethanol metabolism
Fungal Proteins
03 medical and health sciences
chemistry.chemical_compound
Sequence Analysis, Protein
010608 biotechnology
Gene Expression Regulation, Fungal
ethanol production
Genetics
Escherichia coli
Ethanol fuel
Ethanol metabolism
Promoter Regions, Genetic
Gene
030304 developmental biology
Alcohol dehydrogenase
0303 health sciences
Ethanol
Sequence Homology, Amino Acid
Methanol
Alcohol Dehydrogenase
biology.organism_classification
Yeast
Recombinant Proteins
Alcohol oxidase
Culture Media
Alcohol Oxidoreductases
Glucose
chemistry
biology.protein
Biotechnology
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....a7cc3b7703b674c38e9f5bbc5b1e7c5e