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The Nucleoporin Nup153 Has Separable Roles in Both Early Mitotic Progression and the Resolution of Mitosis
- Source :
- Molecular Biology of the Cell. 20:1652-1660
- Publication Year :
- 2009
- Publisher :
- American Society for Cell Biology (ASCB), 2009.
-
Abstract
- Accurate inheritance of genomic content during cell division is dependent on synchronized changes in cellular organization and chromosome dynamics. Elucidating how these events are coordinated is necessary for a complete understanding of cell proliferation. Previous in vitro studies have suggested that the nuclear pore protein Nup153 is a good candidate for participating in mitotic coordination. To decipher whether this is the case in mammalian somatic cells, we reduced the levels of Nup153 in HeLa cells and monitored consequences on cell growth. Reduction of Nup153 resulted in a delay during the late stages of mitosis accompanied by an increase in unresolved midbodies. Depletion of Nup153 to an even lower threshold led to a pronounced defect early in mitosis and an accumulation of cells with multilobed nuclei. Although global nucleocytoplasmic transport was not significantly altered under these depletion conditions, the FG-rich region of Nup153 was required to rescue defects in late mitosis. Thus, this motif may play a specialized role as cells exit mitosis. Rescue of the multilobed nuclei phenotype, in contrast, was independent of the FG-domain, revealing two separable roles for Nup153 in the execution of mitosis.
- Subjects :
- Time Factors
Cell division
Cell Survival
Somatic cell
Mitosis
Biology
Drosophila Proteins
Humans
Kinetochores
Molecular Biology
Kinetochore
Cell growth
Zinc Fingers
Articles
Cell Biology
Cell biology
Nuclear Pore Complex Proteins
Protein Transport
Phenotype
Mitotic exit
Nuclear Pore
RNA Interference
Interphase
Nucleoporin
Gene Deletion
HeLa Cells
Subjects
Details
- ISSN :
- 19394586 and 10591524
- Volume :
- 20
- Database :
- OpenAIRE
- Journal :
- Molecular Biology of the Cell
- Accession number :
- edsair.doi.dedup.....a7e5032ee55c4746395e9139783a45a1
- Full Text :
- https://doi.org/10.1091/mbc.e08-08-0883