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Two membrane juxtaposed signaling modules in ANF-RGC are interlocked
- Source :
- Biochemical and Biophysical Research Communications. 332:149-156
- Publication Year :
- 2005
- Publisher :
- Elsevier BV, 2005.
-
Abstract
- Atrial natriuretic factor (ANF) receptor guanylate cyclase ANF-RGC is a single transmembrane spanning modular protein. Juxtaposed to each side of the transmembrane module is a Cys423-Cys432 disulfide ANF signaling module motif and the ATP-regulated transduction module (ARM) motif. The signaling module motif is conserved in nearly all membrane guanylate cyclases and is believed to be critical in the signaling activities of all membrane guanylate cyclases. The present study with the model system of the olfactory membrane guanylate cyclase shows that this concept is not valid. Furthermore, the study shows that in ANF-GC the signaling motif works through the ARM domain. A new signaling model is proposed where in its natural state the disulfide structural motif represses the ARM domain activity, which, in turn, represses the catalytic module activity of ANF-RGC. ANF signaling relieves the disulfide structural motif restraint on the ARM inhibition and stimulates the catalytic module of the cyclase.
- Subjects :
- GUCY1B3
Biophysics
Biology
Biochemistry
Cyclase
Structure-Activity Relationship
Chlorocebus aethiops
otorhinolaryngologic diseases
Animals
Structural motif
Molecular Biology
GUCY1A2
Binding Sites
Cell Membrane
GUCY1A3
Cell Biology
Guanylate cyclase 2C
NPR1
Transmembrane protein
Cell biology
Guanylate Cyclase
COS Cells
cardiovascular system
Receptors, Atrial Natriuretic Factor
Atrial Natriuretic Factor
hormones, hormone substitutes, and hormone antagonists
Protein Binding
Signal Transduction
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 332
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....a80810a8f81df6db0a7e7f082c2765e0