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Factors associated with obesity alter matrix remodeling in breast cancer tissues
- Source :
- Journal of Biomedical Optics
- Publication Year :
- 2020
- Publisher :
- NLM (Medline), 2020.
-
Abstract
- PubMed: 31983145 Obesity is associated with a higher risk of developing breast cancer and with worse disease outcomes for women of all ages. The composition, density, and organization of the breast tissue stroma are also known to play an important role in the development and progression of the disease. However, the connections between obesity and stromal remodeling are not well understood. We sought to characterize detailed organization features of the collagen matrix within healthy and cancerous breast tissues acquired from mice exposed to either a normal or high fat (obesity inducing) diet. We performed second-harmonic generation and spectral two-photon excited fluorescence imaging, and we extracted the level of collagen-associated fluorescence (CAF) along with metrics of collagen content, three-dimensional, and two-dimensional organization. There were significant differences in the CAF intensity and overall collagen organization between normal and tumor tissues; however, obesity-enhanced changes in these metrics, especially when three-dimensional organization metrics were considered. Thus, our studies indicate that obesity impacts significantly collagen organization and structure and the related pathways of communication may be important future therapeutic targets..
- Subjects :
- Paper
Stromal cell
Matrix remodeling
Gray-Level Co-Occurrence Matrix
Second-Harmonic Generation
Biomedical Engineering
Disease
Matrix (biology)
01 natural sciences
010309 optics
Biomaterials
Mice
Breast cancer
Mammary Glands, Animal
Stroma
Special Section Celebrating Thirty Years of Multiphoton Microscopy in the Biomedical Sciences
0103 physical sciences
Breast Cancer
High fat
Medicine
Animals
Obesity
Three-Dimensional Variance
business.industry
Mammary Neoplasms, Experimental
medicine.disease
Atomic and Molecular Physics, and Optics
3. Good health
Electronic, Optical and Magnetic Materials
Diet
Extracellular Matrix
Mice, Inbred C57BL
Two-Photon Excitation Fluorescence
Microscopy, Fluorescence, Multiphoton
Collagen Fiber Organization
Cancer research
Female
Collagen
Stromal Cells
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Journal :
- Journal of Biomedical Optics
- Accession number :
- edsair.doi.dedup.....a8594710460f0d381a4926b2a0c90b2b