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Distinct genetic profiles of Leishmania (Viannia) braziliensis associate with clinical variations in cutaneous-leishmaniasis patients from an endemic area in Brazil
- Source :
- Parasitology. 145(9)
- Publication Year :
- 2018
-
Abstract
- American tegumentary leishmaniasis (ATL) samples obtained from the lesions of patients with typical (n = 25, 29%), atypical (n = 60, 69%) or both (n = 2%) clinical manifestations were analysed by multilocus enzyme electrophoresis, hsp70 restriction-fragment length polymorphism (PCR-RFLP), hsp70 sequencing and phylogenetics methods. The hsp70 PCR-RFLP analysis revealed two different profiles whose the most samples differed from those expected for Leishmania braziliensis and the other Leishmania species tested: of 39 samples evaluated, two (5%) had a restriction profile corresponding to L. braziliensis, and 37 (95%) had a restriction profile corresponding to a variant pattern. A 1300-bp hsp70 gene fragment was sequenced to aid in parasite identification and a phylogenetic analysis was performed including 26 consensus sequences from the ATL patient's samples and comparing to other Leishmania and trypanosomatids species. The dendrogram allowed to observe a potential population structure of L. braziliensis complex in the studied region, emphasizing that the majority of clinical samples presented a variant genetic profile. Of interest, the L. braziliensis diversity was associated with different clinical manifestations whose parasites with hsp70 variant profile were associated with atypical lesions. The results may be helpful to improve the diagnosis, treatment and control measures of the ATL in endemic areas.
- Subjects :
- 0301 basic medicine
Endemic Diseases
030231 tropical medicine
Hsp70 gene
Leishmaniasis, Cutaneous
Biology
Polymerase Chain Reaction
Leishmania braziliensis
03 medical and health sciences
0302 clinical medicine
Cutaneous leishmaniasis
Phylogenetics
Polymorphism (computer science)
medicine
Humans
HSP70 Heat-Shock Proteins
Phylogeny
Skin
Phylogenetic tree
Dendrogram
Genetic Variation
DNA, Protozoan
medicine.disease
Leishmania
biology.organism_classification
Virology
030104 developmental biology
Infectious Diseases
Animal Science and Zoology
Parasitology
Brazil
Polymorphism, Restriction Fragment Length
Subjects
Details
- ISSN :
- 14698161
- Volume :
- 145
- Issue :
- 9
- Database :
- OpenAIRE
- Journal :
- Parasitology
- Accession number :
- edsair.doi.dedup.....a85cdf1ac9da1f2c8b47c2a26e1a96e0