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Potential Analysis and Preparation of Chitosan Oligosaccharides as Oral Nutritional Supplements of Cancer Adjuvant Therapy
- Source :
- International Journal of Molecular Sciences, Volume 20, Issue 4, International Journal of Molecular Sciences, Vol 20, Iss 4, p 920 (2019)
- Publication Year :
- 2019
- Publisher :
- MDPI, 2019.
-
Abstract
- Cancer is considered to have an adverse influence on health around the world. Chitosan, a linear polysaccharide that contains copolymers of &beta<br />1-4 linked d-glucosamine and N-acetyl-d-glucosamine units, has been widely used in the field of biomedicine, owing to its nontoxicity, biocompatibility, biodegradability, and hemocompatibility. This study was aimed at preparing the chitosan oligosaccharides (COS) and examining its ability on suppressing lung cancer in vitro and in vivo. Human non-small-cell lung cancer A549 cells model and C57BL/6 mice bearing lung cancer model were adopted. COS showed inhibition on the viability and proliferation of lung carcinoma cells (A549) in time-dependent manners, but no cytotoxicity to human liver cell (HL-7702). Moreover, COS could significantly increase Bax expression of A549 cells while decreasing Bcl-2 expression. COS supplementation significantly inhibited the growth of Lewis tissues and promoted necrosis of tumor cells in vivo. After treatment with COS, significantly elevated concentrations of Bax and reduced expression of Bcl-2 in tumor tissues, as well as elevated levels of TNF- &alpha<br />IL-2, Fas and Fas-L in mice serum were observed (p &lt<br />0.05). In conclusion, COS had certain anti-tumor effects and potential application as a synergic functional food ingredient to prevent cancer.
- Subjects :
- 0301 basic medicine
Magnetic Resonance Spectroscopy
Spectrophotometry, Infrared
Cell
Gene Expression
Oligosaccharides
Apoptosis
Pharmacology
lcsh:Chemistry
Mice
0302 clinical medicine
Neoplasms
Cytotoxicity
lcsh:QH301-705.5
Spectroscopy
bcl-2-Associated X Protein
Molecular Structure
Chemistry
General Medicine
Computer Science Applications
medicine.anatomical_structure
Proto-Oncogene Proteins c-bcl-2
030220 oncology & carcinogenesis
chitosan oligosaccharide
cytotoxicity
Cell Survival
Antineoplastic Agents
macromolecular substances
Catalysis
Article
Inorganic Chemistry
03 medical and health sciences
In vivo
Cell Line, Tumor
Adjuvant therapy
medicine
Biomarkers, Tumor
Animals
Humans
Physical and Theoretical Chemistry
Lung cancer
anti-tumor
Molecular Biology
Cell Proliferation
A549 cell
Chitosan
Organic Chemistry
technology, industry, and agriculture
Cancer
medicine.disease
Xenograft Model Antitumor Assays
In vitro
carbohydrates (lipids)
functional
Disease Models, Animal
030104 developmental biology
lcsh:Biology (General)
lcsh:QD1-999
A549 Cells
Dietary Supplements
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 20
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....a868baeb427e16327e4125d70f1155f2