Back to Search
Start Over
Determination of oxytetracycline in marine shellfish (Crassostrea gigas, Ruditapes philippinarum and Scrobicularia plana) by high-performance liquid chromatography using solid-phase extraction
- Source :
- Journal of chromatography. 627(1-2)
- Publication Year :
- 1992
-
Abstract
- A reversed-phase high-performance liquid chromatographic method was developed to detect oxytetracycline (OTC) in three species of marine shellfish (Crassostrea gigas, Ruditapes philippinarum and Scrobicularia plana). Shellfish tissues were analysed after solid-phase extraction by using a mobile phase containing acetonitrile and 0.02 M orthophosphoric acid solution. The linearity and precision of the method were checked over the concentration range 0.100-1.500 micrograms/g. The limits of detection and determination of OTC were 0.040 and 0.100 microgram/g, respectively. The recovery of OTC from spiked shellfish tissues was 79.8%. The OTC concentration in oyster (Crassostrea gigas) spiked at 0.500 microgram/g and stored at -20 degrees C was stable for 6 weeks. The method was applied to a 21-day experimental study performed with oysters.
- Subjects :
- Oyster
animal structures
Ruditapes
Oxytetracycline
Buffers
Biochemistry
High-performance liquid chromatography
Analytical Chemistry
biology.animal
Animals
Solid phase extraction
Scrobicularia plana
Shellfish
Chromatography, High Pressure Liquid
Chromatography
biology
Chemistry
Organic Chemistry
food and beverages
General Medicine
Hydrogen-Ion Concentration
Bivalvia
biology.organism_classification
Kinetics
Mollusca
Environmental chemistry
Crassostrea
Subjects
Details
- Volume :
- 627
- Issue :
- 1-2
- Database :
- OpenAIRE
- Journal :
- Journal of chromatography
- Accession number :
- edsair.doi.dedup.....a86b14c898dda3f2cab3dd31c7d285f0