Back to Search
Start Over
Separation of young and mature thrombocytes by a novel immuno-selection method
- Source :
- Blood cells, moleculesdiseases. 48(3)
- Publication Year :
- 2011
-
Abstract
- Our earlier studies on the structural and functional properties of zebrafish thrombocytes have shown that they have many similarities to mammalian platelets. We have also shown that zebrafish have both young and mature thrombocytes as do mammalian platelets. In addition, we have distinguished young thrombocytes from mature thrombocytes microscopically using lipophilic DiI-C18, and have shown that young thrombocytes have higher GPIIb receptor levels. However, at present, there is no immunoselection method to separate young thrombocytes from mature thrombocytes in order to study differences among them, such as mRNA expression levels of thrombocyte specific genes. We developed a novel technique employing specific biotinylated anti-Cy3 antibody against the chromophore of DiI-C18 and using streptavidin magnetic beads to separate young thrombocytes from mature thrombocytes. Our technique separates and differentiates young and mature thrombocytes from whole blood. This method is specific and is effective with small amounts of blood.
- Subjects :
- Streptavidin
Blood Platelets
Biology
Article
chemistry.chemical_compound
Methylamines
Animals
Platelet
Receptor
Molecular Biology
Zebrafish
Whole blood
Staining and Labeling
Immunomagnetic Separation
Antibodies, Monoclonal
Cell Biology
Hematology
biology.organism_classification
Molecular biology
Cell biology
chemistry
Platelet Glycoprotein GPIb-IX Complex
Biotinylation
biology.protein
Molecular Medicine
RNA
Selection method
Antibody
Subjects
Details
- ISSN :
- 10960961
- Volume :
- 48
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Blood cells, moleculesdiseases
- Accession number :
- edsair.doi.dedup.....a89c5336ee8c98686549bacab5e436f6