Back to Search
Start Over
Antarctic krill (Euphausia superba) in a warming ocean: thermotolerance and deciphering Hsp70 responses
- Source :
- Cell Stress Chaperones, Cell Stress and Chaperones, Cell Stress and Chaperones, Springer Verlag, 2020, 25 (3), pp.519-531. ⟨10.1007/s12192-020-01103-2⟩, Cell Stress and Chaperones, 2020, 25 (3), pp.519-531. ⟨10.1007/s12192-020-01103-2⟩
- Publication Year :
- 2020
-
Abstract
- International audience; The Antarctic krill, Euphausia superba, is a Southern Ocean endemic species of proven ecological importance to the region. In the context of predicted global warming, it is particularly important to understand how classic biomarkers of heat stress function in this species. In this respect, Hsp70s are acknowledged as good candidates. However, previous studies of expression kinetics have not been able to demonstrate significant upregulation of these genes in response to heat shocks at 3 °C and 6 °C for 3 and 6 h. The current work complements these previous results and broadens the prospects for the use of Hsp70s as a relevant marker of thermal shock in this krill species. New experiments demonstrate that induction of Hsp70 isoforms was not detected during exposure to heat shock, but increased expression was observed after several hours of recovery. To complete the analysis of the expression kinetics of the different isoforms, experiments were carried out over short time scales (1 and 2 h at 3 °C and 6 °C) as well as at higher temperatures (9 °C, 12 °C, and 15 °C for 3 h), without any significant response. A 6-week monitoring of animals at 3 °C showed that the time factor is decisive in the establishment of the response. CTmax experiments with incremental times of 1 °C per day or 1 °C every 3 days have shown a particularly high resilience of the animals. The demonstration of the abundance of Hsp70s present before thermal stress in various species of krill, as well as in specimens of E. superba of various origins, showed that the delay in the response in expression could be related to the high constitutive levels of Hsp70 available before the stress experiments. The alternative labelling of the two main isoforms of Hsp70 according to the origin of the animals allowed hypotheses to be put forward on the functioning of thermoregulation in Antarctic krill as well as ice krill.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Thermotolerance
Krill
Hsp70 expression 19
Euphausia
Oceans and Seas
Kril
Zoology
Antarctic Regions
Context (language use)
010603 evolutionary biology
01 natural sciences
Biochemistry
Global Warming
03 medical and health sciences
Abundance (ecology)
[SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN]
Animals
HSP70 Heat-Shock Proteins
14. Life underwater
[SDU.STU.OC]Sciences of the Universe [physics]/Earth Sciences/Oceanography
Original Paper
biology
Global warming
Cell Biology
Thermoregulation
biology.organism_classification
CTmax
heat shock
Euphausia superba
Hsp70
[SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology
030104 developmental biology
Antarctic krill
13. Climate action
[SDV.EE.BIO]Life Sciences [q-bio]/Ecology, environment/Bioclimatology
Hsp70 expression
Euphausiacea
Subjects
Details
- ISSN :
- 14661268 and 13558145
- Volume :
- 25
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Cell stresschaperones
- Accession number :
- edsair.doi.dedup.....a8d75983dc34c79ec8d741dc7fb87b89