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Basic polypeptides as histone models: Circular dichroism of complexes of model polypeptides with DNA

Authors :
Karel Bláha
Ivo Frič
Jaroslav Šponar
Source :
Biophysical Chemistry. 3:255-262
Publication Year :
1975
Publisher :
Elsevier BV, 1975.

Abstract

Circular dichroism (CD) was used to study the complexes of DNA (in 0.15M NaCl) with two polypcptides considered as models of the histonc molecules. CD spectra in the region of DNA absorption were studied with respect to the concentration used for annealing and to the molecular weight and composition of the DNA used. The properties of supernatants after centrifugation of aggregated complexes were examined. The effect of selectively bound antibiotics (actinomycin D and netropsin) on CD spectra of complexes was investigated. The induced CD of proflavine molecules bound to DNA in the various complexes was also studied. It was concluded that changes in the CD spectra of DNA in complexes with the polypeptides are due to the formation of chiral superstructures, even if some conformational changes of DNA molecules themselves may also be decisive in some cases. The superstructure is affected by the composition of DNA. the role of (G + C) rich segments being particularly important.

Details

ISSN :
03014622
Volume :
3
Database :
OpenAIRE
Journal :
Biophysical Chemistry
Accession number :
edsair.doi.dedup.....a8f25eec47ade336f4f486447d279fb0
Full Text :
https://doi.org/10.1016/0301-4622(75)80018-4