Back to Search
Start Over
Basic polypeptides as histone models: Circular dichroism of complexes of model polypeptides with DNA
- Source :
- Biophysical Chemistry. 3:255-262
- Publication Year :
- 1975
- Publisher :
- Elsevier BV, 1975.
-
Abstract
- Circular dichroism (CD) was used to study the complexes of DNA (in 0.15M NaCl) with two polypcptides considered as models of the histonc molecules. CD spectra in the region of DNA absorption were studied with respect to the concentration used for annealing and to the molecular weight and composition of the DNA used. The properties of supernatants after centrifugation of aggregated complexes were examined. The effect of selectively bound antibiotics (actinomycin D and netropsin) on CD spectra of complexes was investigated. The induced CD of proflavine molecules bound to DNA in the various complexes was also studied. It was concluded that changes in the CD spectra of DNA in complexes with the polypeptides are due to the formation of chiral superstructures, even if some conformational changes of DNA molecules themselves may also be decisive in some cases. The superstructure is affected by the composition of DNA. the role of (G + C) rich segments being particularly important.
- Subjects :
- Circular dichroism
Macromolecular Substances
Protein Conformation
Stereochemistry
Biophysics
Biochemistry
Histones
chemistry.chemical_compound
Molecule
Centrifugation
Proflavine
Binding Sites
biology
Circular Dichroism
Organic Chemistry
DNA
Molecular Weight
Kinetics
Crystallography
Histone
chemistry
Netropsin
Dactinomycin
biology.protein
Nucleic Acid Conformation
Spectrophotometry, Ultraviolet
Peptides
Protein Binding
Subjects
Details
- ISSN :
- 03014622
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Biophysical Chemistry
- Accession number :
- edsair.doi.dedup.....a8f25eec47ade336f4f486447d279fb0
- Full Text :
- https://doi.org/10.1016/0301-4622(75)80018-4