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Tetrandrine suppresses human glioma growth by inhibiting cell survival, proliferation and tumour angiogenesis through attenuating STAT3 phosphorylation
- Source :
- European journal of pharmacology. 764
- Publication Year :
- 2015
-
Abstract
- Tetrandrine (Tet), a bisbenzylisoquinoline alkaloid, has been reported to possess anti-tumour activity. However, its effects on human glioma remain unknown. In this study, we demonstrated that Tet inhibited human glioma cell growth in vitro and in vivo. It has been hypothesised that Tet inhibits glioma growth by affecting glioma cell survival, proliferation and vasculature in and around the xenograft tumour in the chick CAM model and signal transducer and activator of transcription 3 (STAT3) mediated these activities. Therefore, we conducted a detailed analysis of the inhibitory effects of Tet on cell survival using a TUNEL assay and flow cytometric analysis; on cell proliferation based on the expression of proliferating cell nuclear antigen; and on angiogenesis using a CAM anti-angiogenesis assay. We used western blotting to investigate the role of STAT3 on the anti-glioma activities of Tet. The results revealed that Tet inhibited survival and proliferation in human glioma cells, impaired tumour angiogenesis and decreased the expression of phosphorylated STAT3 and its downstream proteins. In sum, our data indicate that STAT3 is involved in Tet-induced the regression of glioma growth by activating tumour cell apoptosis, inhibiting glioma cell proliferation and inhibiting angiogenesis.
- Subjects :
- STAT3 Transcription Factor
Angiogenesis
Cell Survival
Neovascularization, Physiologic
Antineoplastic Agents
Chick Embryo
Benzylisoquinolines
Chorioallantoic Membrane
chemistry.chemical_compound
Glioma
Cell Line, Tumor
medicine
Human Umbilical Vein Endothelial Cells
Animals
Humans
Phosphorylation
STAT3
Cell Proliferation
Pharmacology
biology
Neovascularization, Pathologic
Cell growth
Brain Neoplasms
medicine.disease
Proliferating cell nuclear antigen
Tetrandrine
chemistry
Apoptosis
STAT protein
biology.protein
Cancer research
Subjects
Details
- ISSN :
- 18790712
- Volume :
- 764
- Database :
- OpenAIRE
- Journal :
- European journal of pharmacology
- Accession number :
- edsair.doi.dedup.....a9115cd7b06c5051b474f173dfec1a20