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Functionally specialized junctions between endothelial cells of lymphatic vessels
- Source :
- The Journal of experimental medicine, vol 204, iss 10, The Journal of Experimental Medicine
- Publication Year :
- 2007
- Publisher :
- eScholarship, University of California, 2007.
-
Abstract
- Recirculation of fluid and cells through lymphatic vessels plays a key role in normal tissue homeostasis, inflammatory diseases, and cancer. Despite recent advances in understanding lymphatic function (Alitalo, K., T. Tammela, and T.V. Petrova. 2005. Nature. 438:946–953), the cellular features responsible for entry of fluid and cells into lymphatics are incompletely understood. We report the presence of novel junctions between endothelial cells of initial lymphatics at likely sites of fluid entry. Overlapping flaps at borders of oak leaf–shaped endothelial cells of initial lymphatics lacked junctions at the tip but were anchored on the sides by discontinuous button-like junctions (buttons) that differed from conventional, continuous, zipper-like junctions (zippers) in collecting lymphatics and blood vessels. However, both buttons and zippers were composed of vascular endothelial cadherin (VE-cadherin) and tight junction–associated proteins, including occludin, claudin-5, zonula occludens–1, junctional adhesion molecule–A, and endothelial cell–selective adhesion molecule. In C57BL/6 mice, VE-cadherin was required for maintenance of junctional integrity, but platelet/endothelial cell adhesion molecule–1 was not. Growing tips of lymphatic sprouts had zippers, not buttons, suggesting that buttons are specialized junctions rather than immature ones. Our findings suggest that fluid enters throughout initial lymphatics via openings between buttons, which open and close without disrupting junctional integrity, but most leukocytes enter the proximal half of initial lymphatics.
- Subjects :
- Pathology
medicine.medical_specialty
government.form_of_government
Immunology
Biology
Occludin
Inbred C57BL
Medical and Health Sciences
Article
03 medical and health sciences
Mice
0302 clinical medicine
Cell Movement
medicine
Immunology and Allergy
Animals
Lymphocytes
Lymph sacs
Antigens
030304 developmental biology
Lymphatic Vessels
0303 health sciences
Cadherin
Endothelial Cells
Adhesion
Cell Biology
Articles
Cadherins
Endothelial stem cell
Mice, Inbred C57BL
Platelet Endothelial Cell Adhesion Molecule-1
Lymphatic Endothelium
Lymphatic system
030220 oncology & carcinogenesis
government
CD31
Homeostasis
030215 immunology
Subjects
Details
- Database :
- OpenAIRE
- Journal :
- The Journal of experimental medicine, vol 204, iss 10, The Journal of Experimental Medicine
- Accession number :
- edsair.doi.dedup.....a930d75d7da64da15906b4ce875864b8