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Common studied polymorphisms do not affect plasma cytokine levels upon endotoxin exposure in humans

Authors :
Kirsten Møller
Ronan M. G. Berg
Bente Klarlund Pedersen
Helle Bruunsgaard
Sarah Taudorf
K. S. Krabbe
Source :
Clinical and Experimental Immunology. 152:147-152
Publication Year :
2008
Publisher :
Oxford University Press (OUP), 2008.

Abstract

SummaryThe aim of this study was to investigate to what extent single nucleotide polymorphisms (SNPs) in promoter regions of genes of Toll-like receptor (TLR)-4, tumour necrosis factor (TNF)-α, interleukin (IL)-18, interferon (IFN)-γ, IL-6 and IL-10 affect the cytokine response during a controlled low-grade inflammatory response in vivo. Two hundred healthy young male volunteers were genotyped, and cytokine levels were measured in response to a low-dose intravenous bolus of Escherichia coli endotoxin. No association was detected between SNPs (TLR-4299, TLR-4399, TNF-308, IL-18-137, IL-18-607, IFN-γ+874, IL-6-174, IL-10-592 and IL-10-1082) and endotoxin-induced changes in plasma levels of TNF-α, IL-6 and IL-10. IL-18 levels were unaffected by endotoxin. In conclusion, the investigated SNPs did not affect endotoxin-induced low-grade cytokine production of TNF-α, IL-6, IL-18 or IL-10 in healthy young men. Previous reports of a major heritability factor in the inflammatory response may be due to other target genes or effects in older age groups or women.

Details

ISSN :
13652249 and 00099104
Volume :
152
Database :
OpenAIRE
Journal :
Clinical and Experimental Immunology
Accession number :
edsair.doi.dedup.....a95242829759bc337fbf649410793274
Full Text :
https://doi.org/10.1111/j.1365-2249.2008.03612.x