Additional file 2 of Combined transcriptome and proteome profiling of the pancreatic β-cell response to palmitate unveils key pathways of β-cell lipotoxicity

Additional file 2 Supplementary Fig. 2. CREB3L2 deficiency impairs glucose-stimulated insulin secretion. CREB3L2 mRNA expression measured by qRT-PCR in INS-1E cells (A) and human islets (B) exposed to palmitate for 24 h. (C-D) Human islet cells were transfected with CREB3L2 siRNA or control siRNA (siCT) and treated with palmitate for 24 h. (C) Apoptosis evaluated by DNA-binding dyes. (D) CREB3L2 mRNA expression measured by qPCR. (E-G) INS-1E cells were transfected with control siRNA or two Creb3l2 siRNAs. (E) Creb3l2 mRNA expression measured by qPCR. (F) Insulin secretion after incubation with 1.7 mM and 16.7 mM glucose and (G) insulin content following Creb3l2 knockdown. Insulin secretion and content were measured by ELISA and corrected by total protein content. Data are from 4 to 7 independent experiments. *p